M. Scherer, B. Alsdorf, M. Breitbach, K. Matthaei, A. Schnur, N

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Presentation transcript:

Processing challenging forensic casework samples with new protocols for the QIAsymphony SP/AS M. Scherer, B. Alsdorf, M. Breitbach, K. Matthaei, A. Schnur, N. Scholle, L. Bochmann, A. Prochnow, H. Engel Forensic Science International: Genetics Supplement Series Volume 4, Issue 1, Pages e352-e353 (January 2013) DOI: 10.1016/j.fsigss.2013.10.179 Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Comparison of the ADV and standard protocols for DNA extraction from forensic casework samples. DNA yield from a series of saliva (A) and blood (B) dilutions. Forensic Science International: Genetics Supplement Series 2013 4, e352-e353DOI: (10.1016/j.fsigss.2013.10.179) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Comparison of DNA yields from low elution volumes with and without the HE protocol (oil overlay). The HE protocol resulted in a higher DNA yield with both 30 and 50μl elution volumes. Forensic Science International: Genetics Supplement Series 2013 4, e352-e353DOI: (10.1016/j.fsigss.2013.10.179) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Results from normalized STR setup, showing (A) average peak heights across all 16 ESSplex markers and (B) examples of profiles. 4000 RFU is marked in the example profiles as a reference point. Forensic Science International: Genetics Supplement Series 2013 4, e352-e353DOI: (10.1016/j.fsigss.2013.10.179) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions