Synaptic Physiology: Illuminating the Road Ahead Juan Burrone  Current Biology  Volume 15, Issue 21, Pages R876-R878 (November 2005) DOI: 10.1016/j.cub.2005.10.025 Copyright © 2005 Elsevier Ltd Terms and Conditions

Figure 1 Diagram of the FRET response of synapcam 3.1. (A)At rest, YFP and CFP remain far away from each other. Upon stimulation of the nerve and release of glutamate presynaptically, glutamate receptors on the muscle plasma membrane open, allowing calcium influx. Intracellular calcium binds to calmodulin bringing YFP and CFP in close vicinity. (B) In this situation, the excitation of CFP is transferred to YFP, resulting in a decrease in CFP emission and an increase in YFP emission, a process known as FRET. From the ratio of the two signals a FRET signal is obtained. Current Biology 2005 15, R876-R878DOI: (10.1016/j.cub.2005.10.025) Copyright © 2005 Elsevier Ltd Terms and Conditions