NPM1 drives poly-GR into the nucleolus of primary neurons.

Slides:



Advertisements
Similar presentations
Defective phenotypes of ts alp4 and alp6 mutants and the cellular localization of Alp4 and Alp6 at the MTOC. (A) Wild‐type (left, HM123, Table II), alp4‐1891.
Advertisements

Sami Boudkkazi, Aline Brechet, Jochen Schwenk, Bernd Fakler  Neuron 
Poly-GR and poly-PR interact with overlapping proteins in primary neurons and in HEK293 cells. Poly-GR and poly-PR interact with overlapping proteins in.
by Alexis S. Bailey, Shuguang Jiang, Michael Afentoulis, Christina I
STAU1/2 and YBX1 recruit poly-GR/PR into large cytoplasmic granules.
Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients. Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients.
Dynamics of cell–cell repulsion regulated by PYK2 localized at focal adhesions in migrating cells. Dynamics of cell–cell repulsion regulated by PYK2 localized.
Cytoplasmic poly-GR/PR inclusions resemble stress granules in vitro.
Characterization of DPR proteins in primary rat neurons and HEK293 cells. Characterization of DPR proteins in primary rat neurons and HEK293 cells. GFP,
Poly-GR mainly co-localizes with nucleolar ribosomes.
Ang II induces translocation of α-ENaC toward the apical membrane.
Poly-GR and poly-PR interact with similar low-complexity proteins in neurons. Poly-GR and poly-PR interact with similar low-complexity proteins in neurons.
Clone patterns and frequencies produced by lines 168 and 688A.
Reconstituted human Lnp forms stacked discs.
Confocal images of negative controls for Aβ42, Synaptophysin (syp), and PSD95 in hippocampal neurons. Confocal images of negative controls for Aβ42, Synaptophysin.
Volume 16, Issue 12, Pages (June 2006)
The β-tubulin C-terminal tail modulates the coordination of microtubule assembly in an isotype-dependent manner. The β-tubulin C-terminal tail modulates.
Poly-PR inhibits translation in neurons.
Atp1a3 phosphomutants show alterations in subcellular localization.
Arc expression increases H4K12Ac levels.
Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. GFP,
Candidates with a PVM localization.
Fig. 1. Neurobehavioral testing in YG8R mice transplanted with wild-type mouse HSPCs. Neurobehavioral testing in YG8R mice transplanted with wild-type.
The absence of the centrosome changes global microtubule dynamics in a migrating cell. The absence of the centrosome changes global microtubule dynamics.
Marko Kaksonen, Christopher P. Toret, David G. Drubin  Cell 
Axonal swelling and impairment of dendritic development in Purkinje cells from Pex14ΔC/ΔC BL/ICR mouse upon treatment with BDNF. Axonal swelling and impairment.
Marker identification and quantification by StrataQuest, and confocal analysis of EV-containing tissue sections. Marker identification and quantification.
Cholera Toxin FhHDM-1 Merged
KIF18A sNL variants do not accumulate on K-fibers in the center of the spindle. KIF18A sNL variants do not accumulate on K-fibers in the center of the.
Volume 3, Issue 3, Pages (March 2013)
Proteomic Analysis of Mammalian Primary Cilia
Upper cortical layer-enriched neuronal expression of HA-UPRT.
Modification of the βIII-tubulin C-terminal tail does not affect the incorporation of the protein into the microtubule network, the microtubule architecture,
Localization of PBANKA_ and PBANKA_ during blood and liver stage development. Localization of PBANKA_ and PBANKA_ during blood.
Fig. 6 Combination therapy with LVSOD2 and LVshCTGF preserves flap volume and reduces fibrosis after RT. Combination therapy with LVSOD2 and LVshCTGF preserves.
V-SVZ cultures express transcription factors expected for region of origin. V-SVZ cultures express transcription factors expected for region of origin.
Endogenous SMN1 is not recruited to stress granules in HeLa cells after diverse stresses. Endogenous SMN1 is not recruited to stress granules in HeLa cells.
Transcription factors defining dorsal or ventral identity are differentially expressed in dorsal and ventral hamartomas in TSC. Transcription factors defining.
The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.
No correlation between the position of the gene in the nuclear volume and the transcription levels on each of the alleles. No correlation between the position.
PfSec13 does not co-localize with P. falciparum heterochromatin.
Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media. Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and.
Fig. 7. Representative images of control (Cas9+GFP) and Cas9+gRNA+GFP co-injected embryos on day 4 of culture, showing nuclear-imported GFP (green) and.
MET activation increases the density of Syn-GFP/bassoon and Syn-GFP/PSD-95 clusters in neocortical neurons. MET activation increases the density of Syn-GFP/bassoon.
MET/β-catenin complexes during synapse development.
Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and increased T-cell activation. Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and.
ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.
Presence of CGH-1 in the embryo.
Socs1 KD tumors exhibit a more T cell–inflamed phenotype and increased CD8+ T cell activation. Socs1 KD tumors exhibit a more T cell–inflamed phenotype.
Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.
LC8 and its binding partners display broad cellular localization.
Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons. Chd5-deficient NSCs generate excessive astrocytes at the expense of neurons.
The BRCA1 aggregates exclude large nuclear structures.
Association of NM-HA and NM-GFP with SGs is transient.
Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP
Lack of LC3–GFP clustering in neurons expressing mutant CHMP2B.
Effect of M-cadherin RNAi on apoptosis in confluent C2C12 myoblasts.
Subnuclear localization of HA-tagged EBNA1 protein.
PTZ-induced c-Fos and Arc proteins in hippocampal dentate gyrus
Co-expression of VSVG–Cdc42-CA, but not VSVG–Cdc42-DN with eGFP–gephyrin (green) and Myc–CB2ΔPH (blue) rescues postsynaptic gephyrin clustering, as shown.
Septin ring formation and chitin-containing septum formation are aberrant in filaments formed in response to staurosporine. Septin ring formation and chitin-containing.
Knock-out of NSG2 decreases mEPSC frequency.
S-Affs colocalize with SUMO in mammalian cells.
Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered.
PC loss in Nhe6-null mouse cerebellum, female and male.
Purified Vpr induces double-strand breaks in vitro.
Snail-induced multinucleation follows midbody persistence.
Sami Boudkkazi, Aline Brechet, Jochen Schwenk, Bernd Fakler  Neuron 
BDNF protein is expressed in neurons and astrocytes of hippocampus after pilocarpine-induced SE. A, Representative confocal images of hippocampal subfields.
The neuropod cells. The neuropod cells. (Top left) Neuropod cells synapse with sensory neurons in the small intestine, as shown in a confocal microscopy.
Presentation transcript:

NPM1 drives poly-GR into the nucleolus of primary neurons. NPM1 drives poly-GR into the nucleolus of primary neurons. Primary rat neurons (DIV7 + 7) were cotransduced with RFP-tagged nucleolar interactors NPM1 and NOP56 with GFP, GFP-(GR)149, or (PR)175-GFP. (A–C) Single focal planes obtained by confocal microscopy are shown. DAPI was used as nuclear marker and the scale bar depicts 20 μm. RFP was used as negative control. Left columns show GFP signal, middle columns show RFP-tagged proteins, and right columns show merge of GFP, RFP-tagged proteins, and nuclear DAPI staining (blue). RFP-NPM1 and RFP-NOP56 are co-localizing with poly-PR. Note that NPM1 expression recruits poly-GR into the nucleolus (red arrow). Poly-PR–expressing neurons show altered NOP56 nucleolar staining (single channel shown in zoom). (D) Fraction of cells with poly-GR localized to the nucleolus in NPM1– and NOP56–expressing neurons compared with the RFP control (RFP, n = 9; RFP-NPM1, n = 11; and RFP-NOP56, n = 10 images (40× objective) from three independent experiments, mean ± SEM, exact P-values: RFP versus RFP-NPM1, P = 0.0001 and RFP versus RFP-NOP56, P = 0.2307 in one-way ANOVA with Dunnett’s posttest). Hannelore Hartmann et al. LSA 2018;1:e201800070 © 2018 Hartmann et al.