VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang,

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Presentation transcript:

VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang, MD, Wei Sun, MD, Song Han, MD, Chunjiang Yu, MD, Junfa Li, MD, PhD Journal of Vascular Surgery Volume 50, Issue 6, Pages 1452-1458 (December 2009) DOI: 10.1016/j.jvs.2009.08.050 Copyright © 2009 Society for Vascular Surgery Terms and Conditions

Fig 1 Lentivirus-based VCAM-1 siRNA transfection in carotid arteries of rats following CSMDE. Representative images showing no detectable green fluorescence in normal (CON, A) animals but pronounced fluorescence in the neointima of carotid arteries after transfection with lentiviral constructs co-expressing GFP (TRNAS, B); C, Western blotting of carotid arterial homogenates showing the expression of GFP (27 kDa) and β-actin (42 kDa) in CON and TRANS animals; D, Quantitative analysis of Western blot signals shows that GFP expression levels increased significantly in carotid arterial homogenates from TRANS animals (*P < .05 vs CON, n = 6 per group). Journal of Vascular Surgery 2009 50, 1452-1458DOI: (10.1016/j.jvs.2009.08.050) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

Fig 2 siRNA blocks CSMDE-induced VCAM-1 upregulation in rat carotid arteries. A, Representative Western blot showing VCAM-1 (110 kDa) protein expression levels in carotid arteries of rats in the SHAM, CSMDE, CSMDE+CON and CSMDE+RNAi groups. B, Quantitative analysis reveals significant upregulation of VCAM-1 protein expression following CSMDE and CSMDE+CON (*P < .05 vs SHAM). VCAM-1 siRNA significantly reduced VCAM-1 protein expression compared with CSMDE+CON (#P < .05). SHAM, Sham operation; CSMDE, carotid surgical mechanical de-endothelialization; CSMDE+CON, CSMDE+scrambled siRNA; CSMDE+RNAi, CSMDE+VCAM-1 siRNA; n = 10 per group. Journal of Vascular Surgery 2009 50, 1452-1458DOI: (10.1016/j.jvs.2009.08.050) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

Fig 3 Doppler ultrasonographic measurement of rat carotid arteries. A, Representative images showing carotid artery linear diameter and blood velocity measurements; left and right panels respectively show the blood vessel lumens and blood flow spectrograms at the sites of greatest narrowing (arrowheads indicate the points of stenosis). B, Quantitative analysis showing that both arterial linear diameter and blood velocity are significantly decreased in the CSMDE and CSMDE+CON groups (*P < .05 vs SHAM). Treatment of VCAM-1 siRNA transfection (CSMDE+RNAi) resulted in a significant increase in linear diameter and blood velocity (#P < .05 vs CSMDE+CON). n = 10 per group. Journal of Vascular Surgery 2009 50, 1452-1458DOI: (10.1016/j.jvs.2009.08.050) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

Fig 4 Inhibition of carotid artery neointimal hyperplasia by local VCAM-1 siRNA expression in rats following CSMDE. Representative light microscope images of carotid artery cross-sections from SHAM, CSMDE, CSMDE+CON and CSMDE+RNAi animals, scale bar = 200 μm. Journal of Vascular Surgery 2009 50, 1452-1458DOI: (10.1016/j.jvs.2009.08.050) Copyright © 2009 Society for Vascular Surgery Terms and Conditions