Antisense oligonucleotides against the Bcl-2 transcript reversed the cytoprotective effects of DHEA(S) and Allo on serum-deprived PC12 cells. Antisense.

Slides:

Advertisements

Similar presentations

Figure 3. CSE increases the production of IL-8 in A549 cells

Advertisements

Fig. 5. Levels of apoptosis-associated proteins following treatment with parthenolide (PT) and balsalazide. HCT116 cell lysates were prepared after treatment.

E2F7/8 and HIF1 are required for hypoxic VEGFA expression.

MMP-2, MT1-MMP, and TIMP-2 are essential for the invasive capacity of human mesenchymal stem cells: differential regulation by inflammatory cytokines by.

The secreted form of ORF2 (ORF2S) is glycosylated and dimerized.

LPS-Induced Upregulation of SHIP Is Essential for Endotoxin Tolerance

by Madhu Gupta, Paul T. Mungai, and Eugene Goldwasser

Induction of apoptosis by CDIP expression.

S55746 synergizes with panobinostat in vitro and in vivo in DLBCL

FAM49B controls T cell activation by regulating cytoskeleton remodeling. FAM49B controls T cell activation by regulating cytoskeleton remodeling. (A) J.FAM49B.

DHEA(S) and Allo activated transcription factors NF-κB and CREB in serum-deprived PC12 cells. DHEA(S) and Allo activated transcription factors NF-κB and.

IP-MS identifies FAM49B interacting protein Rac.

DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived PC12 cells. DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived.

RGFP-966 decreases HDAC3 activity and is not cytotoxic.

DHEA(S) and Allo induced the expression of the antiapoptotic Bcl-2 proteins in serum-deprived PC12 cells. DHEA(S) and Allo induced the expression of the.

The interaction of FAM49B and Rac.

A model for dual inhibition of BCL2 and MCL1 to release BIM and induce apoptosis. A model for dual inhibition of BCL2 and MCL1 to release BIM and induce.

Bcl-XL down-regulation suppresses the tumorigenic potential of NPM/ALK in vitro and in vivo by Addolorata Maria Luce Coluccia, Silvia Perego, Loredana.

p110-free p85 is present in MEFs and certain murine tissues.

by Rong Chen, Michael J. Keating, Varsha Gandhi, and William Plunkett

DHEA(S) and Allo protected rat chromaffin cells in culture against serum deprivation-induced apoptosis. DHEA(S) and Allo protected rat chromaffin cells.

(A) NK cell cytotoxicity assays against K562, H9, and Raji target cell lines in the presence (black) and absence (white) of an anti-NKp30 antibody, clone.

Thymic phenotype of Themis−/− Shp1 cKO mice is similar to that of Themis−/− mice. Thymic phenotype of Themis−/− Shp1 cKO mice is similar to that of Themis−/−

Phosphatase activity associated with Themis and Shp1 in response to different affinity peptide ligands. Phosphatase activity associated with Themis and.

Themis interacts with Shp2 when Shp1 is genetically deleted.

Serum neutralization against Sabin-1 compared with PV1-RC2010 on different cell culture systems. Serum neutralization against Sabin-1 compared with PV1-RC2010.

I. Kausch, H. Jiang, B. Thode, C. Doehn, S. Krüger, D. Jocham

Phosphatase activity associated with Themis is due to its association with Shp1. Phosphatase activity associated with Themis is due to its association.

Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes. Phosphatase activity associated with Shp1 is reduced in Themis−/− thymocytes.

Molecular Therapy - Nucleic Acids

Figure S1 Nil + CK Nil CK C BIM BCLXL Pro-caspase 3 Cleaved caspase 3

Volume 132, Issue 1, Pages (January 2007)

Rafael Gongora, Robert P Stephan, Zhixin Zhang, Max D Cooper Immunity

Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. Interfering with HMGA2 expression blocks transformation in metastatic.

HPV‐E7 targets RB for induction of ceramide‐dependent mitophagy

Angiotensin II stimulates Pax-2 in rat kidney proximal tubular cells: Impact on proliferation and apoptosis Shao-Ling Zhang, Jun Guo, Babak Moini, Julie.

Peptide release is not impacted by phosphorothioate modification at interface of the P-site and A-site codons. Peptide release is not impacted by phosphorothioate.

USP2a overexpression modifies the microRNA expression profile of prostate cells. USP2a overexpression modifies the microRNA expression profile of prostate.

NsiR4 expression is mediated through an NtcA-activated promoter.

HIPK2 decreases the stability of Notch1-IC through proteasome-dependent degradation. HIPK2 decreases the stability of Notch1-IC through proteasome-dependent.

TRAF4 is required for EGFR activation in response to EGF stimulation.

ZFAND5 expression is induced by dexamethasone, but not by heat shock or tunicamycin treatment. ZFAND5 expression is induced by dexamethasone, but not by.

Figure 2 SORL1 mutants' overexpression increases β-amyloid secretion

Activation of PKR by the PBM decoy peptide.

Distribution of phosphosites and PDGFRα activity in primary cultures.

Susceptibility of rat AECII in primary culture to IAV infection is reduced by rapamycin (mTOR inhibitor) or BGJ398 (FGFR inhibitor). Susceptibility of.

Electrophoretic mobility shift assays for NF-κB in BRP (A) and BREC (B). Electrophoretic mobility shift assays for NF-κB in BRP (A) and BREC (B). Confluent.

Validation of DNMT3A splice variant expression.

Arginine-to-lysine mutations conferring TRIM22 restriction and lysine-to-arginine mutations causing loss of TRIM22 restriction. Arginine-to-lysine mutations.

Fig. 3. Effects of Tec on IL-1β-induced apoptosis in chondrocytes.

miR-145 regulates IGF1R expression.

Ectopic expression of caveolin-1 in ZR75 human breast cancer cells downregulated survivin and decreased cell proliferation. Ectopic expression of caveolin-1.

Aβ-mediated Ras-MAPK signaling and Cyclin D1 expression in B103 cells are dependent on APP expression and can be reversed with MEK or Ras inhibition. Aβ-mediated.

Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional.

Bcl-2 and bcl-xL Antisense Oligonucleotides Induce Apoptosis in Melanoma Cells of Different Clinical Stages Robert A. Olie, Christoph Hafner, Renzo Küttel,

Fig. 3 Proteomic analysis and Western blot analysis of protein cargos of various EVs. Proteomic analysis and Western blot analysis of protein cargos of.

B7-H1–induced reverse signals in myeloma cells.

CDCP1 is required for invadopodia formation and ECM degradation by human breast cancer cells. CDCP1 is required for invadopodia formation and ECM degradation.

TDP1 and TOP1 protein levels correlate positively with TDP1 and TOP1 mRNA levels in colorectal cancer cell lines. TDP1 and TOP1 protein levels correlate.

HNSCC and NSCLC cell lines display differential sensitivities to cixutumumab in the 3D mimic condition. HNSCC and NSCLC cell lines display differential.

Biological effects of BRAF silencing: growth curves of A375 (A) and ARO (B) cell lines in the absence or presence of doxycycline. Biological effects of.

PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.

Identification of the recombinant structural proteins expressed in recombinant baculovirus-infected Sf9 cells by immunofluorescence assay with rabbit anti-FMDV.

The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. Serum-starved.

Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.

Effect of bevacizumab on the proliferation of A2780 cells.

Levels and activity of c-myc are increased in a series of stably transfected DAOY and UW228 medulloblastoma cell lines. Levels and activity of c-myc are.

Effect of BCAA on the progression of cell cycle, expression of p21CIP1, and induction of apoptosis in HepG2 cells in the presence and absence of visfatin.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

Presentation transcript:

Antisense oligonucleotides against the Bcl-2 transcript reversed the cytoprotective effects of DHEA(S) and Allo on serum-deprived PC12 cells. Antisense oligonucleotides against the Bcl-2 transcript reversed the cytoprotective effects of DHEA(S) and Allo on serum-deprived PC12 cells. Cells were cultured for 24 h in serum-free media containing 10–7 M DHEA, DHEAS, or Allo in the absence or the presence of 50 μmol per liter of nonsense (NS) or antisense (AS) 20-mer phosphorothioate oligodeoxynucleotides directed toward the translation-initiation site of the Bcl-2 transcript. Apoptosis was quantified by the APOPercentage assay (A) and the Bcl-2 proteins by Western blot (B). Values represent the mean ± SD of three independent experiments (*, P < 0.05); S, steroid; 1, Bcl-2; 2, actin). Ioannis Charalampopoulos et al. PNAS 2004;101:21:8209-8214 ©2004 by National Academy of Sciences