MicroRNA expression in the human blastocyst

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MicroRNA expression in the human blastocyst Evan M. Rosenbluth, M.D., Dawne N. Shelton, Ph.D., Amy E.T. Sparks, Ph.D., Eric Devor, Ph.D., Lane Christenson, Ph.D., Bradley J. Van Voorhis, M.D. Fertility and Sterility Volume 99, Issue 3, Pages 855-861.e3 (March 2013) DOI: 10.1016/j.fertnstert.2012.11.001 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Confirmed differential expression of miRNAs between euploid and aneuploid embryos by qPCR after normalization to the control probe, snRNA U6, which was consistently expressed in all samples. The vertical axis represents the fold change of the miRNA between the two experimental groups. Error bars represent mean ± SEM; significant fold changes are marked by * (P<.01) and ** (P<.05). Fertility and Sterility 2013 99, 855-861.e3DOI: (10.1016/j.fertnstert.2012.11.001) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Top 20 gene pathways targeted by miRNAs 27b, 141, 339-3p, and 345 predicted by Diana Lab mirPath database and Targetscan 5 web-based software. The vertical bars within each column represent the number of genes targeted by each miRNA within known pathways. The leftmost (blue) vertical bar within each column represents the total number of genes targeted by all four miRNAs in each pathway. Fertility and Sterility 2013 99, 855-861.e3DOI: (10.1016/j.fertnstert.2012.11.001) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions