NG2-CreERT2 postnatal day 31

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NG2-CreERT2 postnatal day 31 Hassouna et al Supplementary Figure 2: EPOR expression in OPC A C 200 µm CA1 CC GFP EPOR DAPI NG2-CreERT2 postnatal day 31 qPCR primary mouse OPC 20 µm GFP DAPI 20 µm GFP EPOR DAPI 20 µm EPOR DAPI B EPOR Antibody (polyclonal rabbit, 400ng/ml) 1. 2. 3. 4. EPOR Western Blot 70- 55- 35- 100- kDa 70- 55- 35- 100- kDa 1. 2. 3. 4. HA Western Blot Protein lysates (15 µg protein/lane): 1: HEK FT cells transfected with murine EPOR 2: EOC-20 cells transduced with HA-tagged human EPOR 3: UT-7 cells (EPO dependent human cell line) 4: Control EOC-20 cells (non-transduced) Bands: Native full length EPOR EPOR fragments