Species differentiation within the Staphylococcus intermedius group using a refined MALDI-TOF MS database J. Murugaiyan, B. Walther, I. Stamm, Y. Abou-Elnaga,

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Species differentiation within the Staphylococcus intermedius group using a refined MALDI-TOF MS database J. Murugaiyan, B. Walther, I. Stamm, Y. Abou-Elnaga, S. Brueggemann-Schwarze, S. Vincze, L.H. Wieler, A. Lübke-Becker, T. Semmler, U. Roesler Clinical Microbiology and Infection Volume 20, Issue 10, Pages 1007-1014 (October 2014) DOI: 10.1111/1469-0691.12662 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 A zoomed view (range from 2000 to 10 500 m/z) of representative matrix-assisted laser desorption ionization—time of flight mass spectra obtained for the distinct members of the Staphylococcus intermedius group (SIG): Staphylococcus delphini (a), Staphylococcus intermedius (b) and Staphylococcus pseudintermedius (c). Clinical Microbiology and Infection 2014 20, 1007-1014DOI: (10.1111/1469-0691.12662) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Graphical representation of the score values obtained for 200 Staphylococcus intermedius group (SIG) isolates before and after refinement (ii) of the Biotyper 3.0 database with reference spectra for 60 SIG together with ten other coagulase-positive staphylococci (= 70 spectra). Circles indicate the score values achieved by applying the manufacturer’s version of the database (approach (i), see Materials and Methods for further explanation) and the diamonds represent the score values reached in the second measurement (ii), associated with a rise of the average value score of 0.24. Clinical Microbiology and Infection 2014 20, 1007-1014DOI: (10.1111/1469-0691.12662) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Comparison of (a) score-oriented dendrogram of matrix-assisted laser desorption ionization—time of flight mass spectral profiles generated using Biotyper 3.0 software and (b) unweighted pair group method with arithmetic mean based tree calculated from the distances of the sequences of the four housekeeping genes pta, cpn60, tuf and 16S rRNA. Clinical Microbiology and Infection 2014 20, 1007-1014DOI: (10.1111/1469-0691.12662) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions