Different types of small nerve fibers in eutopic endometrium and myometrium in women with endometriosis  Natsuko Tokushige, Ph.D., Robert Markham, Ph.D., Peter Russell, M.D., Ian S. Fraser, M.D.  Fertility and Sterility  Volume 88, Issue 4, Pages 795-803 (October 2007) DOI: 10.1016/j.fertnstert.2006.12.078 Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Nerve fibers in eutopic endometrium in women with and without endometriosis. (A) Eutopic endometrium from a woman without endometriosis stained with PGP9.5 and permanent fast red chromogen (magnification, ×200). No nerve fibers were stained. (B) Eutopic endometrium from a woman with endometriosis stained with SP and permanent fast red chromogen (magnification, ×400). Arrows denote tiny SP-positive nerve fibers. (C) Eutopic endometrium from a woman with endometriosis stained with CGRP and permanent fast red chromogen (magnification, ×400). Arrow denotes tiny CGRP-positive nerve fibers. (D) Eutopic endometrium from a woman with endometriosis stained with NPY and permanent fast red chromogen (magnification, ×200). Arrows denote tiny NPY-positive nerve fibers. (E) Eutopic endometrium from a woman with endometriosis stained with VIP and permanent fast red (magnification, ×200). Arrows denote tiny VIP-positive nerve fibers. Bars = 50 μm. Tokushige. Endometrial nerve fibers in women with endometriosis. Fertil Steril 2007. Fertility and Sterility 2007 88, 795-803DOI: (10.1016/j.fertnstert.2006.12.078) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Nerve fibers in myometrium in women with and without endometriosis. (A) Myometrium from a woman without endometriosis stained with TH and permanent fast red chromogen (magnification, ×100). (B) Myometrium from a woman with endometriosis stained with TH and permanent fast red chromogen (magnification, ×100). Arrows denote TH-positive nerve fibers. There was a greater nerve fiber density with much thicker and more obvious nerve trunks in women with endometriosis than in women without endometriosis. Tokushige. Endometrial nerve fibers in women with endometriosis. Fertil Steril 2007. Fertility and Sterility 2007 88, 795-803DOI: (10.1016/j.fertnstert.2006.12.078) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Co-localization of different types of a large nerve trunk in myometrium from a woman with endometriosis (serial sections). (A) Myometrium from a woman with endometriosis stained with SP (magnification, ×200). Nerve fibers were stained with permanent fast red. (B) Myometrium from a woman with endometriosis stained with CGRP (magnification, ×200). Nerve fibers were stained with permanent fast red. (C) Myometrium from a woman with endometriosis stained with TH (magnification, ×200). Nerve fibers were stained with permanent fast red chromogen. (D) Myometrium from a woman with endometriosis stained with VAChT (magnification, ×200). Nerve fibers were stained with permanent fast red chromogen. (E) Myometrium from a woman with endometriosis stained with NPY (magnification, ×200). Nerve fibers were stained with permanent fast red chromogen. (F) Myometrium from a woman with endometriosis stained with VIP (magnification, ×200). Nerve fibers were stained with permanent fast red chromogen. Bars = 50 μm. Tokushige. Endometrial nerve fibers in women with endometriosis. Fertil Steril 2007. Fertility and Sterility 2007 88, 795-803DOI: (10.1016/j.fertnstert.2006.12.078) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions