Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae G. Tzanakaki,

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Presentation transcript:

Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae G. Tzanakaki, M. Tsopanomichalou, K. Kesanopoulos, R. Matzourani, M. Sioumala, A. Tabaki, J. Kremastinou Clinical Microbiology and Infection Volume 11, Issue 5, Pages 386-390 (May 2005) DOI: 10.1111/j.1469-0691.2005.01109.x Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Examples of the results obtained when the multiplex PCR assay was applied to clinical samples, showing detection of Haemophilus influenzae type b (181 bp), Neisseria meningitidis (110 bp) and Streptococcus pneumoniae (80 bp). Lanes: 1, φX174 HaeIII digest; 2, positive control for all three targets; 3–13, clinical samples from blood (lanes 3, 6, 10, 11) or CSF (lanes 4, 5, 7, 8, 9, 12 and 13); 14, negative control. Clinical Microbiology and Infection 2005 11, 386-390DOI: (10.1111/j.1469-0691.2005.01109.x) Copyright © 2005 European Society of Clinical Infectious Diseases Terms and Conditions