HAEMOPHILUS & BORDETELLA Lecture 11 Prepared by: Miss Norzawani Jaffar Bsc (Hons) Biomedical Sciences, UKM

Learning Outcomes At the end of learning session, student must be; Able to describe the morphological characteristic for Haemophilus and Bordetella Differentiate Haemophilus and Bordetella Understand and able to explain the laboratory test for species identification of Haemophilus and Bordetella

Haemophilus sp. Common Characteristic.

Heamophilus influenzea

Laboratory Test X and V test

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Bordetella spp.

Flagellated B. bronchiseptica

Laboratory Test PCR assay for B.pertussis Direct-fluoresent-antibody (DFA) testing using reagents (polyclonal and monoclonal) Positive DFA result must be come together with PCR confirmation or culture for B. pertussis. Culture method – role in isolation of B. pertussis and related species, especially for antimicrobial susceptibility testing (AST) in cases of treatment failure.

Specimen Collection Timing;

2. Collection of nasopharyngeal specimen

End…. Q & a