Sysmex XE-2100 NRBC Detection.

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Presentation transcript:

Sysmex XE-2100 NRBC Detection

NRBC Counts - Methodology From semiconductor laser flow cytometry. STROMATOLYSER-NR (diluent) STROMATOLYSER-NR dye (stain) Passed through the laser flow cytometer for analysis. Counted using side fluorescence and forward light scatter.

Flow Cytometry Forward light scatter - cell size Side fluorescence - RNA/DNA information (nucleus)

NRBC Channel Explained

Therefore the NRBC Scattergram…

Resolution of NRBC and WBC Clear & reliable separation of NRBC and WBC Wbc take upp more dye than NRBC - so higher fluorescence Smaller volume of the NRBC nucleus gives reduced forward light scatter.

CORRECTING WBC COUNT

The NRBC Count Algorithm NRBC% = NRBC# x 100 NRBC# + WBC# (NRBC/ 100 WBC) WBC# NRBC#

The Corrected WBC Count Eo Ba Mo Ne Ly NRBC NRBC

NRBC # Revised Algorithm WBC region in WBC/Baso and Diff scattergram include NRBC if NRBC appear

WBC & DIFF channels: Correction for NRBC’s Correct WBC Minus Correct WBC Differential Minus

NRBC Flags Swiching Algorithm NRBC-CH is measured “NRBC present” flag is monitored (WBC abnormal flag category) Detection from “NRBC%” NRBC-CH is not measured “NRBC?” flag is monitored (WBC suspect flag category) Detection from DIFF scattergram