Altered microRNA expression in stenoses of native arteriovenous fistulas in hemodialysis patients Lei Lv, MD, Weibin Huang, MD, Jiwei Zhang, MD, Yaxue.

Slides:

Advertisements

Similar presentations

Down-regulated expression of family with sequence similarity 3, member B (FAM3B), in oral squamous cell carcinoma Masashi Shiiba, Shunsaku Ishige, Yasuhiro.

Advertisements

MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers Thomas X. Lu,

Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small.

Identification of synovial fluid microRNA signature in knee osteoarthritis: differentiating early- and late-stage knee osteoarthritis Y.-H. Li, G. Tavallaee,

Volume 141, Issue 3, Pages (March 2012)

Skin Pharmacol Physiol 2017;30: DOI: /

MicroRNAs Involved in the Mitogen-Activated Protein Kinase Cascades Pathway During Glucose-Induced Cardiomyocyte Hypertrophy E. Shen, Xuehong Diao, Xiaoxia.

Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia Go Kuwahara,

Chung S. Lim, MRCS, PhD, Serafim Kiriakidis, PhD, Ewa M

The effects of doxycycline and micronized purified flavonoid fraction on human vein wall remodeling are not hypoxia-inducible factor pathway-dependent

M. Fu, G. Huang, Z. Zhang, J. Liu, Z. Zhang, Z. Huang, B. Yu, F. Meng

MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes S.J. Park, E.J. Cheon,

Cell Physiol Biochem 2015;36: DOI: /

Group IIa secretory phospholipase expression correlates with group IIa secretory phospholipase inhibition–mediated cell death in K-ras mutant lung cancer.

Differential Expression of Circular RNAs in Glioblastoma Multiforme and Its Correlation with Prognosis Junle Zhu, Jingliang Ye, Lei Zhang, Lili Xia,

MicroRNA-30b is a multifunctional regulator of aortic valve interstitial cells Mi Zhang, MD, Xiaohong Liu, MD, Xiwu Zhang, MD, Zhigang Song, MD, Lin Han,

Chung S. Lim, MRCS, PhD, Serafim Kiriakidis, PhD, Ewa M

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps Rahmi Oklu,

Nicotine Induces Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor by α1 Nicotinic Acetylcholine Receptor–Mediated Activation in.

Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats Zhenhai Yu, MD, Keisuke.

MicroRNA-92a-3p regulates the expression of cartilage-specific genes by directly targeting histone deacetylase 2 in chondrogenesis and degradation G.

Kenneth M. DeSart, MD, Khayree Butler, MD, Kerri A

MicroRNAs in spent blastocyst culture medium are derived from trophectoderm cells and can be explored for human embryo reproductive competence assessment

MicroRNA-92a-3p regulates the expression of cartilage-specific genes by directly targeting histone deacetylase 2 in chondrogenesis and degradation G.

An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb.

Yongping Shao, Kaitlyn Le, Hanyin Cheng, Andrew E. Aplin

Characterization of microRNA transcriptome in tumor, adjacent, and normal tissues of lung squamous cell carcinoma Jun Wang, MD, PhD, Zhi Li, MD, PhD,

Kristopher G. Maier, PhD, Xuan Han, Benjamin Sadowitz, MD, Karen L

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk

Urokinase-induced smooth muscle cell responses require distinct signaling pathways: A role for the epidermal growth factor receptor Suzanne M. Nicholl,

MicroRNA signature in patients with eosinophilic esophagitis, reversibility with glucocorticoids, and assessment as disease biomarkers Thomas X. Lu,

Potential role of circulating microRNAs as a biomarker for unexplained recurrent spontaneous abortion Weibing Qin, M.D., Ph.D., Yunge Tang, M.D., Ning.

Brahma-related gene 1 inhibits proliferation and migration of human aortic smooth muscle cells by directly up-regulating Ras-related associated with diabetes.

Circulating anti-β2-glycoprotein I antibodies of peripheral arterial disease patients trigger a genomic overexpression of Toll-like receptor 4 in endothelial.

Corona cell RNA sequencing from individual oocytes revealed transcripts and pathways linked to euploid oocyte competence and live birth Jason C. Parks,

MicroRNA-381 Represses ID1 and is Deregulated in Lung Adenocarcinoma

Inflammation-related induction of absent in melanoma 2 (AIM2) in vascular cells and atherosclerotic lesions suggests a role in vascular pathogenesis

MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes S.J. Park, E.J. Cheon,

A microRNA profile comparison between thoracic aortic dissection and normal thoracic aorta indicates the potential role of microRNAs in contributing to.

Transforming growth factor-β increases vascular smooth muscle cell proliferation through the Smad3 and extracellular signal-regulated kinase mitogen-activated.

Identification of synovial fluid microRNA signature in knee osteoarthritis: differentiating early- and late-stage knee osteoarthritis Y.-H. Li, G. Tavallaee,

Postmortem and ex vivo carbon monoxide ventilation reduces injury in rat lungs transplanted from non–heart-beating donors Boming Dong, MD, PhD, Paul.

Α1-Adrenergic receptors mediate combined signals initiated by mechanical stretch stress and norepinephrine leading to accelerated mouse vein graft atherosclerosis

Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia Sébastien Déglise, MD, David Martin, MS, Hervé.

Differential expression of elastin assembly genes in patients with Stanford Type A aortic dissection using microarray analysis Bernice L.Y. Cheuk, PhD,

Volume 20, Issue 2, Pages (January 2010)

Proinflammatory role of stem cells in abdominal aortic aneurysms

A novel vascularized patch enhances cell survival and modifies ventricular remodeling in a rat myocardial infarction model Qi Zhou, MD, PhD, Jian-Ye.

Nicotine Induces Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor by α1 Nicotinic Acetylcholine Receptor–Mediated Activation in.

MiR-146a Negatively Regulates TLR2-Induced Inflammatory Responses in Keratinocytes Florian Meisgen, Ning Xu Landén, Aoxue Wang, Bence Réthi, Charbel.

Simvastatin Protects Human Melanocytes from H2O2-Induced Oxidative Stress by Activating Nrf2 Yuqian Chang, Shuli Li, Weinan Guo, Yuqi Yang, Weigang Zhang,

Volume 85, Issue 2, Pages (January 2014)

Transcriptomic analysis of patients with tetralogy of Fallot reveals the effect of chronic hypoxia on myocardial gene expression Mohamed T. Ghorbel,

MicroRNA-101 Exerts Tumor-Suppressive Functions in Non-small Cell Lung Cancer through Directly Targeting Enhancer of Zeste Homolog 2 Ji-guang Zhang,

Leptin receptor is elevated in carotid plaques from neurologically symptomatic patients and positively correlated with augmented macrophage density Jacob.

Volume 79, Issue 7, Pages (April 2011)

Volume 23, Issue 8, Pages (August 2015)

MiR-125b, a MicroRNA Downregulated in Psoriasis, Modulates Keratinocyte Proliferation by Targeting FGFR2 Ning Xu, Petter Brodin, Tianling Wei, Florian.

Molecular Mechanisms Regulating the Defects in Fragile X Syndrome Neurons Derived from Human Pluripotent Stem Cells Tomer Halevy, Christian Czech, Nissim.

The effects of doxycycline and micronized purified flavonoid fraction on human vein wall remodeling are not hypoxia-inducible factor pathway-dependent

Volume 9, Issue 5, Pages (November 2017)

Molecular Therapy - Nucleic Acids

Evidence for Altered Wnt Signaling in Psoriatic Skin

In vitro differences between smooth muscle cells derived from varicose veins and normal veins Ying Xiao, PhD, Zhibin Huang, MD, Henghui Yin, PhD, Ying.

MicroRNA-381 Represses ID1 and is Deregulated in Lung Adenocarcinoma

Long Noncoding RNA BC as a Novel Therapeutic Target for Colorectal Cancer that Suppresses Metastasis by Upregulating TIMP3 Jiaxin Lin, Xin Tan,

Molecular Therapy - Nucleic Acids

Protein Kinase C-Dependent Upregulation of miR-203 Induces the Differentiation of Human Keratinocytes Enikö Sonkoly, Tianling Wei, Elizabeth Pavez Loriè,

MicroRNA-22-3p is down-regulated in the plasma of Han Chinese patients with premature ovarian failure Yujie Dang, M.D., Ph.D., Shidou Zhao, Ph.D., Yingying.

Circular RNA Transcriptomic Analysis of Primary Human Brain Microvascular Endothelial Cells Infected with Meningitic Escherichia coli Ruicheng Yang,

Presentation transcript:

Altered microRNA expression in stenoses of native arteriovenous fistulas in hemodialysis patients Lei Lv, MD, Weibin Huang, MD, Jiwei Zhang, MD, Yaxue Shi, MD, Lan Zhang, MD Journal of Vascular Surgery Volume 63, Issue 4, Pages 1034-1043.e3 (April 2016) DOI: 10.1016/j.jvs.2014.10.099 Copyright © 2016 Society for Vascular Surgery Terms and Conditions

Fig 1 MicroRNA (miRNA) profiles differentiate the stenosed vein segments of primary arteriovenous fistulas (AVFs) from the control segments. A, Heatmap of aberrantly expressed miRNAs in the stenosed vein segments of primary AVF and the controls. Colors indicate relative signal intensities: red and green colors indicate upregulated and downregulated miRNAs, respectively. B, Volcano plot demonstrates the differences in expression levels of miRNAs between stenosed vein segments of primary AVFs and the control veins. The log-fold change was plotted against the log odds of differential expression using the R package limna (The R Foundation for Statistical Computing, http://www.r-project.org/foundation). The vertical lines correspond to twofold up and down, respectively, and the horizontal line represents a P value = .05. The red point in the plot represents the differentially expressed miRNAs with statistical significance. A volcano plot provided further information about the significance and magnitude of expressive alteration of selected miRNAs. Journal of Vascular Surgery 2016 63, 1034-1043.e3DOI: (10.1016/j.jvs.2014.10.099) Copyright © 2016 Society for Vascular Surgery Terms and Conditions

Fig 2 Verification of selected microarray data by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Triplicate assays were done for each RNA sample, and the relative amount of each microRNA (miRNA) was normalized to U6 small nuclear RNA. Values are expressed as mean ± standard deviation. *P < .05 indicates statistically significant difference between the two groups. A, Upregulated miRNAs in the stenosed vein segments of primary arteriovenous fistulas (AVFs). B, Downregulated miRNAs in the stenosed vein segments of primary AVFs. Journal of Vascular Surgery 2016 63, 1034-1043.e3DOI: (10.1016/j.jvs.2014.10.099) Copyright © 2016 Society for Vascular Surgery Terms and Conditions

Fig 3 The microRNA gene ontology (GO) network. GO function enrichment was performed by mapping the predicted target genes from the microRNAs (miRNAs) to the GO databases. By enrichment analyses on the predicted targets predicted from the significantly validated most differentially expressed miRNAs, we determined that the target genes could be categorized into a large number of significant GO terms. The major target genes in the biologic process categories were involved in cellular process, protein binding, regulation of biologic process, RNA biosynthetic process, regulation of metabolic process, and cell proliferation, among others. The circles represent genes and the squares represent miRNAs. The center of the network was represented by degree. The term degree refers to the contribution of one miRNA to the GOs around or the contribution one GO to the miRNAs around. There were just a few predicted target genes for hsa-miR-3960, the miRNA therefore was absent in the network. Journal of Vascular Surgery 2016 63, 1034-1043.e3DOI: (10.1016/j.jvs.2014.10.099) Copyright © 2016 Society for Vascular Surgery Terms and Conditions

Fig 4 The pathway network. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment was performed by mapping the predicted target genes from the microRNAs (miRNAs) to the KEGG databases. By enrichment analyses on the targets predicted from the significantly validated most differentially expressed miRNAs, we determined that the target genes could be categorized into many regulatory pathways. Enrichment ranking of KEGG pathways include mitogen-activated protein kinase (MAPK) signaling pathway, Wnt signaling pathway, phosphatidylinositol 3-kinase-Akt signaling pathway, gap junction, focal adhesion, p53 signaling pathway, apoptosis, and pathways in cancer, among others. The red circle represents pathways. The relationship of the pathway is represented by an arrow. The center of the network is represented by degree. The term degree refers to the number of interaction relationship of pathways. The pathway network could summarize the pathways interaction of differential expression genes under diseases. Journal of Vascular Surgery 2016 63, 1034-1043.e3DOI: (10.1016/j.jvs.2014.10.099) Copyright © 2016 Society for Vascular Surgery Terms and Conditions

Fig 5 Validation for the expression of c-Jun NH2-terminal kinase (JNK2), extracellular signal-regulated kinase 5 (ERK5), growth-factor receptor-bound protein 2 (GRB2), and protein phosphatase 3 calcineurin A-α (PPP3CA) in the vessels by Western blot analysis. Representative blots are shown in A, C, E, and G. Bands were quantified by densitometric analysis, and the results are shown as relative density compared with control in B, D, F, and H. Values expressed as mean ± standard deviation from three independent experiments. *P < .05 indicates statistically significant difference between the two groups. JNK2, ERK5, GRB2, and PPP3CA were overexpressed in the stenotic arteriovenous fistula (AVF) group compared with control veins. Journal of Vascular Surgery 2016 63, 1034-1043.e3DOI: (10.1016/j.jvs.2014.10.099) Copyright © 2016 Society for Vascular Surgery Terms and Conditions

Fig 6 Validation for the expression of c-Jun NH2-terminal kinase (JNK2), extracellular signal-regulated kinase 5 (ERK5), growth-factor receptor-bound protein 2 (GRB2), and protein phosphatase 3 calcineurin A-α (PPP3CA) in the vessels by immunohistochemistry analysis. Representative photomicrographs are shown at original magnification ×200. The brown color staining represents cellular JNK2, ERK5, GRB2, and PPP3CA expression. Enhanced JNK2, ERK5, GRB2, and PPP3CA staining was observed in the stenotic AVF group. Journal of Vascular Surgery 2016 63, 1034-1043.e3DOI: (10.1016/j.jvs.2014.10.099) Copyright © 2016 Society for Vascular Surgery Terms and Conditions

Supplementary Fig 1 (online only) The micro RNA (miRNA)-gene ontology (GO) network. The circles represent genes and the squares represent miRNAs. Their relationship is represented by one edge. The center of the network represents the degree, the interaction of one miRNA with the GOs around and the interaction of one GO with the miRNAs around. The key miRNAs and genes in the network always have the highest degrees. There were just a few predicted target genes for hsa-miR-3960, the miRNA therefore was absent in the network. Journal of Vascular Surgery 2016 63, 1034-1043.e3DOI: (10.1016/j.jvs.2014.10.099) Copyright © 2016 Society for Vascular Surgery Terms and Conditions

Supplementary Fig 2 (online only) Expression of c-Jun NH2-terminal kinase (JNK2), extracellular signal-regulated kinase 5 (ERK5), growth-factor receptor-bound protein 2 (GRB2), and protein phosphatase 3 calcineurin A-α (PPP3CA) in the vessels by real-time polymerase chain reaction (PCR). Values are expressed as mean ± standard deviation from three independent experiments. *P < .05 indicates statistically significant difference between the two groups. In the stenotic arteriovenous fistula (AVF) group compared with controls, the messenger RNA (mRNA) expression of JNK2, ERK5, GRB2, and PPP3CA was significantly increased. Journal of Vascular Surgery 2016 63, 1034-1043.e3DOI: (10.1016/j.jvs.2014.10.099) Copyright © 2016 Society for Vascular Surgery Terms and Conditions