Maria J. Worsham, Sandra R. Wolman, Richard J. Zarbo

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Presentation transcript:

Molecular Approaches to Identification of Tissue Contamination in Surgical Pathology Sections Maria J. Worsham, Sandra R. Wolman, Richard J. Zarbo The Journal of Molecular Diagnostics Volume 3, Issue 1, Pages 11-15 (February 2001) DOI: 10.1016/S1525-1578(10)60643-5 Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 A: A routine H&E section of an endoscopic gastric biopsy from an elderly male with diffuse gastric mucosal erythema and pebbling, labeled Case A. B: A routine H&E section of a gastric biopsy from a female whose endoscopic examination was highly suggestive of malignant ulcer (contaminant), labeled Case B. C: FISH on tissue section derived from the gastric biopsy illustrated in A. Note the presence of one red signal (X chromosome) and one green signal (Y chromosome) in gastric mucosal cells.D: FISH on the tissue section derived from the gastric biopsy shown in B(contaminant). Note the presence of two red signals (representing X chromosomes) and absence of green signals. The Journal of Molecular Diagnostics 2001 3, 11-15DOI: (10.1016/S1525-1578(10)60643-5) Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 A: H&E section from Case C composed of benign prostatic epithelium. B: H&E section from Case D showing moderately to poorly differentiated prostatic adenocarcinoma, with Gleason pattern 3 + 5 (Gleason score = 8), and with perineural invasion. C:Autoradiograph of microsatellite analysis of DNA from Case C and Case D (putative source of contaminant). Lane 1: DNA-1 microdissected from the H&E-stained tissue section area of alleged contaminant labeled Case D present on the slide from Case C. Lane 2: DNA-1′ microdissected from tissue sections of a paraffin block of prostate adenocarcinoma from which Case D was suspected to originate. Lane 3: DNA-2 microdissected from the H&E-stained tissue section area of normal prostatic epithelium from Case C.Lane 4: DNA-2′ microdissected from tissue sections originating from a paraffin block of Case C. The Journal of Molecular Diagnostics 2001 3, 11-15DOI: (10.1016/S1525-1578(10)60643-5) Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions