Announcements Today: Applications of DNA: FISH, DNA “chips”, Forensics

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Presentation transcript:

Announcements Today: Applications of DNA: FISH, DNA “chips”, Forensics Wednesday– quiz on Chpt 1 of ECB (not Chpt 2) HW #2, Due on Wednesday Today: Applications of DNA: FISH, DNA “chips”, Forensics Next time: Fundamental studies: bending & twisting rigidity of DNA with Magnetic Traps.

Green- # 22 marker- 22q13 Red- DiGeorge Syndrome region (if missing) at 22q11.2 (Person has 2 normal)  http://members.aol.com/chrominfo/metafish.htm

FISH A B C A). Chromosone 4 “painted”. B) From same person in A, but hybridized with a probe for the terminal part of chromosome 4q. Only one green signal  one chromosome 4 is missing material from the terminal end of 4q. C) Xcen chromosome 22 Other: Steroid Sulfatase gene Two X chromosomes, 1 St.Su. gene  female carrier for Steroid Sulfatase Deficiency. http://members.aol.com/chrominfo/metafish.htm

Temin & Baltimore in 1970:1975 Nobel Prize DNA polymerase Modification of Dogma DNA polymerase  Temin & Baltimore in 1970:1975 Nobel Prize Reverse Transcriptase: ssRNA ssDNA Important example of this? a) HIV-AIDS viral infection b) Telomerase (ends of chromosomes) R.T. (normal process) Replicates “poorly”: lots of errors. 1 per 15-30 kbases; compared to 1 in 109 for DNA polymerase No proofreading, unlike DNA polymerase. AIDS uses this cleverly. How?

Nature Genetics Supplement 21:33-37 (January 1999) (PDF 1M)

DNA Forensics Every person has their own, unique DNA (except for twins). A person can be “tagged” with their DNA. If your blood, semen is found you’re in trouble. Your genes found in a kid  parent. You’re killed in war/car accident and can only recognize you from your DNA. Don’t have to completely sequence their DNA. Can find certain regions. Just enough –say 13 different ones– that chances that another person has exactly the same set is 1 in a trillion.