In Vitro Fertilization May Increase the Risk of Beckwith-Wiedemann Syndrome Related to the Abnormal Imprinting of the KCNQ1OT Gene Christine Gicquel,

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In Vitro Fertilization May Increase the Risk of Beckwith-Wiedemann Syndrome Related to the Abnormal Imprinting of the KCNQ1OT Gene Christine Gicquel, Véronique Gaston, Jacqueline Mandelbaum, Jean-Pierre Siffroi, Antoine Flahault, Yves Le Bouc The American Journal of Human Genetics Volume 72, Issue 5, Pages 1338-1341 (May 2003) DOI: 10.1086/374824 Copyright © 2003 The American Society of Human Genetics Terms and Conditions

Figure 1 Methylation analysis of KvDMR1 in liver tissue (patient 131) and leukocytes (patients 15, 94, 98, 115, and 137) from the six patients with BWS born after ART and in leukocytes from a normal control (C). Genomic DNA was digested with BamHI and the methylation-sensitive enzyme NotI. Digested samples were subjected to electrophoresis in a 0.7% agarose gel, blotted onto Hybond XL membranes, and hybridized with the HLHAY79 KvDMR1 probe corresponding to EST 68627 (ATCC; Manassas). The upper band (6 kb) is methylated and corresponds to the maternal allele. The lower band (4.2 kb) is unmethylated and corresponds to the paternal allele. The American Journal of Human Genetics 2003 72, 1338-1341DOI: (10.1086/374824) Copyright © 2003 The American Society of Human Genetics Terms and Conditions