Marked increase in incidence of Achromobacter xylosoxidans infections caused by sporadic acquisition from the environment Winnie Ridderberg, Karen E.M.

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Marked increase in incidence of Achromobacter xylosoxidans infections caused by sporadic acquisition from the environment Winnie Ridderberg, Karen E.M. Bendstrup, Hanne V. Olesen, Søren Jensen-Fangel, Niels Nørskov-Lauritsen Journal of Cystic Fibrosis Volume 10, Issue 6, Pages 466-469 (December 2011) DOI: 10.1016/j.jcf.2011.07.004 Copyright © 2011 European Cystic Fibrosis Society Terms and Conditions

Fig. 1 Pulsed field gel electrophoresis of SpeI digested genomic DNA from 15 isolates of A. xylosoxidans collected during 2009 from 15 CF patients. Five patients had been infected for more than six years (a–e), five patients had been infected for two to three years (f–j), and five patients experienced a first-time identification of A. xylosoxidans in 2009 (k–o). Four patients carry strains with identical macro restriction profiles (c–f). Lanes M: molecular marker. Journal of Cystic Fibrosis 2011 10, 466-469DOI: (10.1016/j.jcf.2011.07.004) Copyright © 2011 European Cystic Fibrosis Society Terms and Conditions

Fig. 2 Pulsed field gel electrophoresis of SpeI digested genomic DNA from twenty two isolates of A. xylosoxidans cultured from airway secretions from a CF patient between 2003 and 2009. Lanes 1–2: 2003, 3–6: 2005, 7–10: 2006, 11–14: 2007, 15–18: 2008, 19–22: 2009. Lanes M: molecular marker. Journal of Cystic Fibrosis 2011 10, 466-469DOI: (10.1016/j.jcf.2011.07.004) Copyright © 2011 European Cystic Fibrosis Society Terms and Conditions

Fig. 3 Pulsed field gel electrophoresis of SpeI digested genomic DNA from isolates of A. xylosoxidans. A. Isolates from a CF patient who underwent lung transplantation in 2005. B. Isolates from a CF patient who underwent lung transplantation in 2006. Lanes M: molecular marker. Journal of Cystic Fibrosis 2011 10, 466-469DOI: (10.1016/j.jcf.2011.07.004) Copyright © 2011 European Cystic Fibrosis Society Terms and Conditions