Volume 127, Issue 2, Pages (August 2004)

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Volume 127, Issue 2, Pages 493-501 (August 2004) Lack of immunological or molecular evidence for a role of mouse mammary tumor retrovirus in primary biliary cirrhosis Carlo Selmi, Susan R. Ross, Aftab A. Ansari, Pietro Invernizzi, Mauro Podda, Ross L. Coppel, M.Eric Gershwin Gastroenterology Volume 127, Issue 2, Pages 493-501 (August 2004) DOI: 10.1053/j.gastro.2004.05.033

Figure 1 Nucleotide sequences of MMTV and human betaretroviruses, including Genbank accession numbers, with PCR amplification primers used for virus detection in liver samples. Gastroenterology 2004 127, 493-501DOI: (10.1053/j.gastro.2004.05.033)

Figure 2 A representative Western blot showing reactivities against MMTV-C3H virions (molecular weights are indicated). The viral preparation was separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with either representative goat anti-p27 (lane 1), anti-MMTV (lane 2), or anti-gp52 (lane 3) polyclonal antibodies; serum from an AMA-positive patient with PBC (dilution 1:200, lane 4); and rabbit anti-lipoic acid polyclonal antibody (lane 5). Note that the PBC serum in lane 4 reacts against 2 (57- and 74-kilodalton) lipoylated proteins that are not recognized by any of the antiviral polyclonal antibodies. Gastroenterology 2004 127, 493-501DOI: (10.1053/j.gastro.2004.05.033)

Figure 3 Immunohistochemistry of MMTV-infected mouse mammary tumor (MMT) and PBC and control liver sections using unconjugated goat polyclonal anti-MMTV or anti-gp52 sera. Alexa 488–conjugated (green fluorescence) anti-goat IgG was used as secondary antibody. At least one bile duct is shown for each liver specimen. The MMT section presents a clear positive pattern with both antibodies, whereas no signal can be detected within sections from PBC or control. (Original magnification 400×.) Gastroenterology 2004 127, 493-501DOI: (10.1053/j.gastro.2004.05.033)

Figure 4 PCR analysis of DNA obtained from non-PBC (lanes A-F) and PBC (lanes 34, 41, 51, 53, 55, and 56) liver specimens. Results with each set of primers (including the positive control hTfR1) used are shown for all human samples, no DNA (lane N), mouse genomic DNA (lane M), and cloned MMTV proviral DNA (lane +). Gastroenterology 2004 127, 493-501DOI: (10.1053/j.gastro.2004.05.033)

Figure 5 PCR analysis of DNA obtained from 10 PBC and 10 control PBL samples. Results (including the positive control hTfR1) are shown for all human samples, no DNA (lane N), and mouse genomic DNA spiked with 50 pg cloned MMTV proviral DNA (lane +). Gastroenterology 2004 127, 493-501DOI: (10.1053/j.gastro.2004.05.033)