Reading Gels -once a gel has been run, it is stained with another chemical exposed to UV light to allow the DNA to appear -below is an example of a gel.

Slides:



Advertisements
Similar presentations
General Microbiology Lecture Twelve Identification of Bacteria
Advertisements

Chapter E4.1 Living things inherit traits in patterns.
Chapters 13 and 14-Genetic Engineering and the Human Genome $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Minipulation The Newest Technology.
Genetic Engineering Genetic Engineers can alter the DNA code of living organisms. Selective Breeding Recombinant DNA Gel Electrophoresis Transgenic Organisms.
Traits & Environment Pp What are traits? Hair color Eye color HeightWeight Male vs. Female.
Collect Buccal Cells. PCR Polymerase Chain Reaction DNA/gene amplification.
Sex Linked Traits Humans have 23 pairs of chromosomes.
Chapter 13 Section 1 DNA Technology. DNA Identification Only.10% of the human genome varies from person to person 98% of our genetic makeup does not code.
Genetic Engineering Genetic Engineers can alter the DNA code of living organisms. Selective Breeding Recombinant DNA PCR Gel Electrophoresis Transgenic.
Advances since Watson & Crick
From the Gene to the Genome Genetic Inheritance Patterns Observing Genetic Differences in the DNA.
Genetics and Inheritance Year 10 Biology Part 1: Genes & Chromosomes.
Electrophoresis. A process that is used to sort fragments of DNA by placing the digested DNA in a special gel and adding electricity.
Comparing DNA Aim: How can we compare organisms using gel electrophoresis? Do Now: Write two observations from your results of gel electrophoresis.
Section 12.3 Complex Inheritance of Human Traits
Genes Traffic lights quiz Hold up the coloured card that matches the correct answer you see on the screen.
Compete revision sheet Allele= One member of a pair or series of genes that occupy a specific position on a specific chromosome Dominant = gene that.
DNA Profiling (DNA fingerprinting) pard/cleared.html.
Essential Question: How are traits passed on from the parents to their offspring?
Living things inherit traits in patterns
Electrophoresis 7 th Grade,. Learning Objectives Understanding how electrophoresis facilitates the separation of molecules Be familiar with the types.
The C3HC4-Type RING Zinc Finger and MYB Transcription Factor Families Matthew Taube June 5, 2008 HC70AL.
DNA marker analysis Mrs. Stewart Medical Interventions Central Magnet School.
DNA Fingerprinting Techniques
Medical Science I.  Community  Group of populations that live together in a defined area (Ex: businesses, people, pets, etc. in Alvin)  Population.
Current Genetic Techniques How can we use DNA today? Section 3 - Parts of Chapters 13 & 14.
HOW DOES DNA WORK? Section 4.2. Vocabulary RNA: RiboNucleic Acid... A molecule that is present in all living cells and plays an important role in protein.
How Do Traits Get Passed On? LESSON 4. Move to which corner you think is correct for each question Can offspring get instructions for the variation of.
PCR Polymerase chain reaction. PCR is a method of amplifying (=copy) a target sequence of DNA.
Biotechnology  Biotechnology involves human manipulation of the genetic code.  Genetic engineering is the process of manipulating genes for practical.
DNA & B IOTECHNOLOGY. Biotechnology Recombinant DNA Gene splicing Restriction enzymes Sticky ends Ligase & DNA-ase DNA sequencing Gene probes DNA profiling.
Genetic fingerprinting
Lab 8: PCR (Polymerase Chain Reaction)
(II) Manipulating DNA A. Isolating a specific DNA segment
Lec 1 Molecular biology.
LT- Today, I can apply concepts that I have learned about DNA and solve a crime by drawing evidence from a DNA video, informational text, and DNA fingerprints.
Receipts’ Blood Receipts’ Bone Marrow Receipt’s Spleen
CHROMOSOMES.
How traits are passed from parents to offspring.
Try not to leave any behind – I dare you!
How DNA Works section 2.
Genetic Engineering Genetic Engineers can alter the DNA code of living organisms. Selective Breeding Recombinant DNA PCR Gel Electrophoresis Transgenic.
Gel Electrophoresis.
Advances since Watson & Crick chemheritage
DNA Part 2.
DNA FINGER PRINTING.
How are areas of DNA that don’t code for proteins (genes) used by our cells? How can we make use of these areas?
Bellwork: What is the human genome project. What was its purpose
Lab 8: PTC Polymerase Chain Reaction Lab
Genetics Definitions Definition Key Word
محاضرة عامة التقنيات الحيوية (هندسة الجينات .. مبادئ وتطبيقات)
Multiple Alleles Sex Linked Gene Carrier Genetic Disorder
THE HUMAN GENOME Molecular Genetics.
Simulated Lab Relationships & Biodiversity
Simulated Lab Relationships & Biodiversity
DNA ELECTROPHORESIS OR DNA FINGERPRINTING.
Heredity Vocabulary Sexual Reproduction- a cell containing genetic info from the mother and a cell containing genetic info from the father combine into.
Title of Notes p. 9 RS DNA Fingerprinting.
EVOLUTION? Modern Clues Martin.
DNA Fingerprinting Gel Electrophoresis.
Simulated Lab Relationships & Biodiversity
Chapter 13: Biotechnology
Cell Divisions & Mutations
Variable Number Tandem Repeats (VNTR)
Levels of Organization
Quick genetics review.
Bellringer: What does the term “biotechnology” mean?
Simulated Lab Relationships & Biodiversity
Notes: DNA Fingerprinting pg. 3-4
Genetic Engineering.
Presentation transcript:

Reading Gels -once a gel has been run, it is stained with another chemical exposed to UV light to allow the DNA to appear -below is an example of a gel showing various different samples of DNA and how they compare -notice how some bands are located in the same line on the DNA; those indicate the size of the DNA and they are recorded as base pairs (or bp)

Reading Gels -this process of running and reading a gel has been used to link family members where family members will have similar bands as compared to unrelated people -this process is also used for identification purposes since each person will have a unique banding pattern -for this lab, we are looking for an Alu element present on a particular gene in our DNA (on chromosome 16); this gene codes for a protein that dissolves blood clots -the Alu element exists throughout the DNA and seems to help in the secretion of proteins from the cell -the phenotypes are either all positive (present) for the element, positive and negative for the element, or all negative (not present)

Example Gel Directions: Make some observations about the gels below: