Peanut defensins: Novel allergens isolated from lipophilic peanut extract Arnd Petersen, PhD, Skadi Kull, PhD, Sandra Rennert, MSc, Wolf-Meinhard Becker,

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Peanut defensins: Novel allergens isolated from lipophilic peanut extract Arnd Petersen, PhD, Skadi Kull, PhD, Sandra Rennert, MSc, Wolf-Meinhard Becker, PhD, Susanne Krause, PhD, Martin Ernst, PhD, Thomas Gutsmann, PhD, Johann Bauer, PhD, Buko Lindner, PhD, Uta Jappe, MD, MSc Journal of Allergy and Clinical Immunology Volume 136, Issue 5, Pages 1295-1301.e5 (November 2015) DOI: 10.1016/j.jaci.2015.04.010 Copyright © 2015 The Authors Terms and Conditions

Fig 1 Two-dimensional PAGE of hydrophobic interaction chromatography flow through of the lipophilic peanut extract. Separation was performed under reducing conditions and Coomassie stained. The protein spot under consideration is indicated. M, Molecular mass marker; pI, isoelectric point. Journal of Allergy and Clinical Immunology 2015 136, 1295-1301.e5DOI: (10.1016/j.jaci.2015.04.010) Copyright © 2015 The Authors Terms and Conditions

Fig 2 A, SDS-PAGE of the isolated protein fraction separated under reducing (R) and nonreducing (N) conditions with Coomassie staining. B and C, Western blots for identification of IgE-reactive sera from patients with peanut allergy under nonreducing (Fig 2, B) and reducing (Fig 2, C) conditions. C, Buffer control; I, India ink staining; M, molecular mass marker; P, patients' sera (numbers as listed in Table I); P*, nonallergic subject. Journal of Allergy and Clinical Immunology 2015 136, 1295-1301.e5DOI: (10.1016/j.jaci.2015.04.010) Copyright © 2015 The Authors Terms and Conditions

Fig 3 The basophil activation test (CD203c) was performed in blood samples of 4 patients with peanut allergy. Blood samples were stimulated with 0.0054 to 5400 μg/L peanut defensin or 4.37 to 437.55 μg/L formyl-methionyl-leucyl-phenylalanine (fMLP) as a positive control. For unstimulated samples, PBS was used. MFI, Mean fluorescence intensity; PE, phycoerythrin. Journal of Allergy and Clinical Immunology 2015 136, 1295-1301.e5DOI: (10.1016/j.jaci.2015.04.010) Copyright © 2015 The Authors Terms and Conditions

Fig 4 Determination of antifungal activity by using a growth inhibition assay of different fungal species analyzed in the presence of the peanut defensin. Journal of Allergy and Clinical Immunology 2015 136, 1295-1301.e5DOI: (10.1016/j.jaci.2015.04.010) Copyright © 2015 The Authors Terms and Conditions

Fig 5 A, Comparison of the 3 peanut defensins detected by using SDS-PAGE under nonreducing conditions. Complete protein sequences were deduced from the EST database. N-terminal protein sequences are underlined. Sequence comparison is presented as identity in percentages. Tryptic peptide fragments were analyzed by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and are highlighted in gray. MW, Molecular weight. B, Electrospray ionization–Fourier transform ion cyclotron resonance mass spectrum of the isolated protein fraction. Monoisotopic neutral masses are indicated. Journal of Allergy and Clinical Immunology 2015 136, 1295-1301.e5DOI: (10.1016/j.jaci.2015.04.010) Copyright © 2015 The Authors Terms and Conditions

Fig E1 Western blot experiments with 14 additional subjects (for characterization, see Table E1) under nonreducing conditions.E4 C, Buffer control; I, India ink staining; M, molecular mass marker; P, patients' sera (numbers are as listed in Table E1); P4, positive control patient (see Table I); TCE, protein staining with trichloroethanol. Journal of Allergy and Clinical Immunology 2015 136, 1295-1301.e5DOI: (10.1016/j.jaci.2015.04.010) Copyright © 2015 The Authors Terms and Conditions

Fig E2 Additional basophil activation tests (CD203c) were performed with 2 patients with peanut allergy, 5 patients with allergy but not peanut allergy, and 1 nonallergic nonsensitized subject. Blood samples were stimulated with 0.0054 to 5,400 μg/L peanut defensin or 4.37 to 437.55 μg/L formyl-methionyl-leucyl-phenylalanine (fMLP) or 5,000 to 500,000 μg/L zymosan as a positive control. For unstimulated samples, PBS was used. MFI, Mean fluorescence intensity; PE, phycoerythrin. Journal of Allergy and Clinical Immunology 2015 136, 1295-1301.e5DOI: (10.1016/j.jaci.2015.04.010) Copyright © 2015 The Authors Terms and Conditions

Fig E3 Sequence alignment of selected plant defensins. Rs-AFP2 is the radish (Raphanus sativus) plant defensin.E9 Defensins from the Leguminosae include Ara h 12-13 (peanut defensins), pea (Pisum sativum), Gly m 2 from Glycine maxima (only the N-terminal sequence is knownE10). Plant allergens with defensin-like domains include Art v 1 from Artemisia vulgarisE11 and Amb a 4 from Ambrosia artemisiifolia.E12 Amino acids conserved in plant defensins are indicated in red; mostly conserved amino acids are in blue. Journal of Allergy and Clinical Immunology 2015 136, 1295-1301.e5DOI: (10.1016/j.jaci.2015.04.010) Copyright © 2015 The Authors Terms and Conditions