Volume 130, Issue 7, Pages 2001-2009 (June 2006) Estrogen Receptor α Polymorphisms Associated With Susceptibility to Hepatocellular Carcinoma in Hepatitis B Virus Carriers  Yun Zhai, Gangqiao Zhou, Guohong Deng, Weimin Xie, Xiaojia Dong, Xiumei Zhang, Ling Yu, Hao Yang, Xiaoyan Yuan, Hongxin Zhang, Lianteng Zhi, Zhijian Yao, Yan Shen, Boqing Qiang, Fuchu He  Gastroenterology  Volume 130, Issue 7, Pages 2001-2009 (June 2006) DOI: 10.1053/j.gastro.2006.02.030 Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions

Figure 1 LD measures of the ESR1 polymorphisms in our study population. (A) Gene structure and positions of the 6 ESR1 polymorphisms investigated in this study. (B) LD statistics for ESR1 gene. The upper right triangle plot provides the absolute values of the D′; the lower left triangle plot provides a measure of the correlation coefficient, r2. Gastroenterology 2006 130, 2001-2009DOI: (10.1053/j.gastro.2006.02.030) Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions

Figure 2 Allele-specific expression difference of ESR1 messenger RNA. (A) The generation of the standard curve. The log2 of (VIC intensity/FAM intensity) for ESR1 was plotted against log2 of (VIC allele/FAM allele) of homozygous DNAs at 7 different ratios (8:1, 4:1, 2:1, 1:1, 1:2, 1:4, and 1:8; VIC allele/FAM allele). (B) The allelic gene expression ratios for ESR1 in several types of samples. FLC, fetal liver cell (n = 3); UBMC, umbilical blood mononuclear cell (n = 4); APBMC, adult peripheral blood mononuclear cells (n = 6). ⁎Allelic difference in gene expression is significantly deviated from equal expression (P < .05). Gastroenterology 2006 130, 2001-2009DOI: (10.1053/j.gastro.2006.02.030) Copyright © 2006 American Gastroenterological Association Institute Terms and Conditions