Saeid Fathollahi 1*,Dr. Farah Farokhi 1,Dr. Golamreza Najafi 2 The Study Protective Role of Spirulina platensis against Oxidative stress caused by Glyphosate in Male Rats Testicle. Saeid Fathollahi 1*,Dr. Farah Farokhi 1,Dr. Golamreza Najafi 2 1. Department of Biology, Faculty of Basic Sciences, Urmia University, Urmia, Iran 2. Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Objectives Materials & Methods Results Glyphosate, is a broad spectrum herbicide widely used to eliminate unwanted plants. Glyphosate was toxic for human embryonic cells, induced Sperm abnormalities and oxidative stress. Spirulina platensis (S) can reduce oxidative stress, testicular damage, and sperm abnormalities by its potent antioxidant activity. This study was conducted to determine the protective role of Spirulina platensis against Oxidative stress caused by Glyphosate. 36 adult male rats were randomized into six experimental groups(n=6). group1 received 0.20 ml normal saline(control), group2 received 250mg/kg Glyphosate, group3 received500mg/kg Glyphosate, group4 received 500mg/kg S, group5 received 500mg/kg S+250mg/kg Glyphosate, group6 received500 mg/kg S+500mg/kg Glyphosate. All administration were done orally. After 56 days, Malondialdehyde (MDA) values and Total antioxidant capacity (TAOC) were measured by the Chessman-Esterbauer and FRAP method, respectively. The data indicated that Glyphosate groups had significant reduction of TAOC, and significant increase in MDA values compared with control group. Spirulina platensis co-administration significantly (P<0.05) improves the reduction of TAOC caused by glyphosate, it also reduces MDA levels. Figure 2. The concentration of Total Antioxidant capacity (TAOC) in control and different groups; Different superscripts show significant differences between groups. G250: 250mg/kg/bw of glyphosate, G500: 500mg/kg/bw of glyphosate, sp: spirulina platensis Conclusion Figure 1 . The concentration of malondialdehyde(MDA) in control and different groups; All numbers are mean + standard error, Different superscripts show significant differences between groups. G250: 250mg/kg/bw of glyphosate, G500: 500mg/kg/bw of glyphosate, sp: spirulina platensis, Spirulina platensis can reduce oxidative stress by its potent antioxidant activity and noticeably can improve adverse effects of Glyphosate on mail reproduction system. Corresponding Email: saeidfth1990@gmail.com