Lysyl oxidase expression in a rat model of arterial balloon injury

Slides:

Advertisements

Similar presentations

Zoledronate Inhibits Intimal Hyperplasia in Balloon-injured Rat Carotid Artery L. Wu, L. Zhu, W.H. Shi, B. Yu, D. Cai European Journal of Vascular and.

Advertisements

Inhibition of intimal thickening after vascular injury with a cocktail of vascular endothelial growth factor and cyclic Arg-Gly-Asp peptide Yue Li, Lucinda.

Decellularized vein as a potential scaffold for vascular tissue engineering Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M.

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps Rahmi Oklu,

Xue Ma, MD, Jeffrey D. Pearce, MD, David B. Wilson, MD, William P

Low molecular weight (LMW) heparin inhibits injury-induced femoral artery remodeling in mouse via upregulating CD44 expression Gaofeng Zhao, MD, PhD,

Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential Lorenzo Boni, MD,

Inhibitory effects of mesenchymal stem cells in intimal hyperplasia after balloon angioplasty Ae-Kyeong Kim, PhD, Min-Hee Kim, Do-Hyung Kim, Ha-Nl Go,

Anti–VLA-4 antibody reduces intimal hyperplasia in the endarterectomized carotid artery in nonhuman primates Alan B. Lumsden, MB, ChB, Changyi Chen,

Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries Jens W Fischer,

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9 Ada W.Y. Chung, PhD, Pooja.

Loss of STAT1 is associated with increased aortic rupture in an experimental model of aortic dissection and aneurysm formation Matthew J. Eagleton, MD,

Gene transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel- coated polyester stent graft Hongshan Zhong, MD, Osamu Matsui, MD,

Randolph L. Geary, MD, Seppo T. Nikkari, MD, William D. Wagner, PhD, J

The cyclolignan picropodophyllin attenuates intimal hyperplasia after rat carotid balloon injury by blocking insulin-like growth factor-1 receptor signaling

Gene transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel- coated polyester stent graft Hongshan Zhong, MD, Osamu Matsui, MD,

True extracranial carotid artery aneurysm in a child

Lipoplex gene transfer of inducible nitric oxide synthase inhibits the reactive intimal hyperplasia after expanded polytetrafluoroethylene bypass grafting

Lucas P. Neff, MD, Bryan W. Tillman, MD, PhD, Saami K

Interleukin 18 binding protein (IL18-BP) inhibits neointimal hyperplasia after balloon injury in an atherosclerotic rabbit model Jian-Ming Li, MD, Mohammad.

Adenovirus-mediated intra-arterial delivery of cellular repressor of E1A-stimulated genes inhibits neointima formation in rabbits after balloon injury

Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia Sébastien Déglise, MD, David Martin, MS, Hervé.

Vida K. Stark, MS, Thomas F. Warner, MD, John R. Hoch, MD

Differential expression of elastin assembly genes in patients with Stanford Type A aortic dissection using microarray analysis Bernice L.Y. Cheuk, PhD,

Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Decellularized vein as a potential scaffold for vascular tissue engineering Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M.

Ian S. Zagon, PhDa, Frank M. Essis, MSa, Michael F

Influence of endothelial cell seeding on platelet deposition and patency in small- diameter Dacron arterial grafts Brent T. Allen, M.D., Julie A. Long,

Aging exacerbates neointimal formation, and increases proliferation and reduces susceptibility to apoptosis of vascular smooth muscle cells in mice Roberto.

Recombinant human thrombomodulin inhibits arterial neointimal hyperplasia after balloon injury Jian-ming Li, MD, Michael J Singh, MD, Mazen Itani, MD,

Tissue factor pathway inhibitor-2 is induced by fluid shear stress in vascular smooth muscle cells and affects cell proliferation and survival Johan.

Alex Westerband, MD, Joseph L. Mills, MD, John M. Marek, MD, Ronald L

Nitric oxide–releasing biopolymers inhibit thrombus formation in a sheep model of arteriovenous bridge grafts Paul S. Fleser, MD, Vijay K. Nuthakki,

Magnetic nanosphere-guided site-specific delivery of vascular endothelial growth factor gene attenuates restenosis in rabbit balloon-injured artery Tiemin.

S-phase kinase-associated protein-2 (Skp2) promotes vascular smooth muscle cell proliferation and neointima formation in vivo Yih-Jer Wu, MD, PhD, Graciela.

Spatiotemporal expression and localization of matrix metalloproteinas-9 in a murine model of thoracic aortic aneurysm Jeffrey A. Jones, PhD, John R.

Leptin receptor is elevated in carotid plaques from neurologically symptomatic patients and positively correlated with augmented macrophage density Jacob.

Nick D. Tsihlis, PhD, Jozef Murar, BA, Muneera R. Kapadia, MD, Sadaf S

Antisense basic fibroblast growth factor gene transfer reduces early intimal thickening in a rabbit femoral artery balloon injury model David G. Neschis,

Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins Xavier Berard, MD, PhD, Sébastien Déglise, MD, Florian Alonso, PhD,

Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype Hector F. Simosa, MD, Grace Wang, MD, XinXin Sui,

Antisense basic fibroblast growth factor gene transfer reduces neointimal thickening after arterial injury Abigail K. Hanna, MD, Jonathan C. Fox, MD,

Delayed inhaled carbon monoxide mediates the regression of established neointimal lesions Michael Madigan, MD, Fateh Entabi, MD, Brian Zuckerbraun, MD,

Marcel Scheinman, MD, Enrico Ascher, MD, Gabriel S

Inhibition of experimental neointimal hyperplasia by recombinant human thrombomodulin coated ePTFE stent grafts Geoffrey Wong, MD, Jian-ming Li, MD,

Gene expression changes evoked in a venous segment exposed to arterial flow Deborah Abeles, MD, Stephanie Kwei, MD, George Stavrakis, MS, Yuzhi Zhang,

Perivascular delivery of losartan with surgical fibrin glue prevents neointimal hyperplasia after arterial injury Michael C Moon, MD, Katerina Molnar,

The role of short-term oxygen administration in the prevention of intimal hyperplasia Charu Lata, MBBS, Derrick Green, MD, Jing Wan, MD, Sabita Roy, PhD,

Carotid plaque instability and ischemic symptoms are linked to immaturity of microvessels within plaques Benjamin J. Dunmore, PhD, Mark J. McCarthy,

Association of smooth muscle cell phenotypic modulation with extracellular matrix alterations during neointima formation in rabbit vein grafts Wei-da.

Yehuda G. Wolf, MD, Lars M. Rasmussen, MD, Yoav Sherman, MD, Warner P

Hemodynamic unloading leads to regression of pulmonary vascular disease in rats Stacy B. O'Blenes, MD, MSca, Stefan Fischer, MDb, Brendan McIntyre, BSca,

Neointimal hyperplasia on a cell-seeded polytetrafluoroethylene graft is promoted by transfer of tissue plasminogen activator gene and inhibited by transfer.

Volume 59, Issue 6, Pages (June 2001)

Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study Wangde Dai,

Integrin αvβ3 as a target in the prevention of neointimal hyperplasia

Macrophage depletion reduces monocyte chemotactic protein-1 and transforming growth factor-β1 in healing rat vein grafts Randal A Wolff, PhD, Jeffrey.

Michael L. Marin, MD, Ronald E. Gordon, PhD, Frank J

Nick D. Tsihlis, PhD, Jozef Murar, BA, Muneera R. Kapadia, MD, Sadaf S

Short-term dexamethasone treatment inhibits vein graft thickening in hypercholesterolemic ApoE3Leiden transgenic mice Abbey Schepers, MD, Nuno M.M. Pires,

Controlled release of small interfering RNA targeting midkine attenuates intimal hyperplasia in vein grafts Hiroshi Banno, MD, Yoshifumi Takei, PhD,

Sidhu P. Gangadharan, MD, Mohammad H. Eslami, MD, Inanna P

Differential transcriptional activation of matrix metalloproteinase-2 and membrane type-1 matrix metalloproteinase by experimental deep venous thrombosis.

Adenoviral-mediated uteroglobin gene transfer inhibits neointimal hyperplasia after balloon injury in the rat carotid artery Robert A. Larson, MD, Mina.

Angiotensin II induces histomorphologic features of unstable plaque in a murine model of accelerated atherosclerosis Valdeci da Cunha, PhD, Baby Martin-McNulty,

Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats Chih-Yang Chan, MD, Yih-Sharng Chen, MD,

Endoluminal smooth muscle cell seeding limits intimal hyperplasia

Prevention of stenosis after vascular reconstruction: Pharmacologic control of intimal hyperplasia—A review Alexander W. Clowes, MD, Michael A. Reidy,

Gene expression changes evoked in a venous segment exposed to arterial flow Deborah Abeles, MD, Stephanie Kwei, MD, George Stavrakis, MS, Yuzhi Zhang,

Presentation transcript:

Lysyl oxidase expression in a rat model of arterial balloon injury Vijay K. Nuthakki, MD, Paul S. Fleser, MD, Lauren E. Malinzak, MD, Marilyn L. Seymour, BS, Rose E. Callahan, MS, Phillip J. Bendick, PhD, Gerald B. Zelenock, MD, Charles J. Shanley, MD Journal of Vascular Surgery Volume 40, Issue 1, Pages 123-129 (July 2004) DOI: 10.1016/j.jvs.2004.02.028

Fig 1 Steady-state lysyl oxidase mRNA expression after balloon injury of the rat carotid artery or sham operation. Injured right and uninjured left common carotid arteries were harvested at 0, 0.25, 1, 3, 7, 14, 21, 28, 60, and 120 days after injury; sham-operated right and uninjured left common carotid arteries were harvested at 0, 3, 7, 14, 21, and 28 days after injury, and lysyl oxidase expression was evaluated with TaqMan reverse transcription polymerase chain reaction. In balloon-injured arteries levels were elevated to 184% of those in the contralateral uninjured artery by 3 days after injury (P = .0133, one-way analysis of variance). Elevated mRNA expression continued in a broad peak until 21 days, after which expression levels returned to and below those in the contralateral uninjured artery through 120 days. Data are expressed as mean ± SEM; n = 5 animals for all time points except day 3 (n = 8) and day 21 (n = 7). Expression is not significantly altered in sham-operated rat carotid arteries compared with contralateral control arteries. Data are expressed as mean ± SEM; n = 2 animals at each time point. There is a statistically significant increase in mean lysyl oxidase expression in balloon-injured vs sham-operated rat carotid arteries (P =.0160, one-way analysis of variance). Journal of Vascular Surgery 2004 40, 123-129DOI: (10.1016/j.jvs.2004.02.028)

Fig 2 Qualitative determination of lysyl oxidase expression in balloon-injured and contralateral uninjured rat carotid artery over time. Immunohistochemical staining of representative sections with a custom polyclonal antibody to lysyl oxidase and the labeled streptavidin biotin technique with Nova Red chromogen and hematoxylin counterstaining. Positive staining for lysyl oxidase in the intima is seen at the luminal edge and within the smooth muscle cell–containing layers of the hyperplastic intima at days 14 and 28 after injury (arrows). By 60 days only a scant amount of lysyl oxidase protein expression is seen in the intima. A minimal amount of constitutive protein expression is found surrounding the smooth muscle cells in the media of all sections, including control uninjured arteries. (All images, original magnification ×40.) Journal of Vascular Surgery 2004 40, 123-129DOI: (10.1016/j.jvs.2004.02.028)

Fig 3 Two-color immunohistochemical localization of lysyl oxidase and α-smooth muscle cell actin in balloon-injured rat carotid artery 28 days after injury. Immunostaining for α-smooth muscle cell actin was with a monoclonal antibody and Nova Red chromogen (red; white arrows). Immunostaining for lysyl oxidase was with a custom rabbit polyclonal antibody and diaminobenzidene chromogen (black; black arrows). Most cells in the neointima expressed α-smooth muscle cell actin, but lysyl oxidase was most strongly expressed by α-actin–positive cells primarily adjacent to the luminal surface. Lysyl oxidase expression in the media was mainly restricted to the perinuclear area of some actin-positive cells. (Original magnification ×40.) Journal of Vascular Surgery 2004 40, 123-129DOI: (10.1016/j.jvs.2004.02.028)

Fig 4 Mean maximal intimal area after balloon injury of the rat carotid artery. Intimal area was calculated with the Optimas image processing system from digitized photographs of hematoxylin and eosin–stained paraffin-embedded sections of rat carotid arteries. The injured arteries from all groups showed measurable intimal thickening at day 7, with an early increase in cellularity and maximal intimal area at day 28. Neointimal area decreased considerably by 60 days after injury, and approached baseline values by 120 days. The intimal area differed significantly across different days (P < .0001, two-way analysis of variance). Data are presented as mean intimal areas ± SEM; n = 5 animals for all time points except days 3, 7, and 21, where n = 7. Journal of Vascular Surgery 2004 40, 123-129DOI: (10.1016/j.jvs.2004.02.028)

Fig 5 Qualitative determination of changes in cellular composition of the vessel wall and matrix formation in balloon-injured rat carotid artery over time. Movat pentachrome stains nuclei and elastin fibers black; cell cytoplasm and muscle red; actin fibers intense red; proteoglycan, ground substance, and mucin blue; and collagen yellow. Increasing amounts of red staining, reflecting smooth muscle proliferation, are seen in the hyperplastic intimal layer of injured arteries over time through day 21 after injury. Total collagen is increased at days 21 and day 28. By days 60 and 120, as intimal area decreases collagen staining is reduced and increased blue staining is seen, reflecting the presence of proteoglycan and ground substance. Red staining is visible in the media in all sections, because of the presence of smooth muscle cells. (All images, original magnification ×40.) Journal of Vascular Surgery 2004 40, 123-129DOI: (10.1016/j.jvs.2004.02.028)