Novel rapid PCR for the detection of Ile491Phe rpoB mutation of Mycobacterium tuberculosis, a rifampicin-resistance-conferring mutation undetected by.

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Novel rapid PCR for the detection of Ile491Phe rpoB mutation of Mycobacterium tuberculosis, a rifampicin-resistance-conferring mutation undetected by commercial assays E. André, L. Goeminne, A. Colmant, P. Beckert, S. Niemann, M. Delmee Clinical Microbiology and Infection Volume 23, Issue 4, Pages 267.e5-267.e7 (April 2017) DOI: 10.1016/j.cmi.2016.12.009 Copyright © 2016 The Author(s) Terms and Conditions

Fig. 1 (a) Design of the multiplex allele-specific PCR for the detection of the Ile491Phe rpoB rifampicin-resistance mutation. The rpoB_I491_F primer is specific to the wild-type profile, whereas the rpoB_F and rpoB_R primers are universal. (b) Results: the method allowed the correct identification of the rpoB Ile491Phe mutation among the 39/78 rifampicin-resistant strains. Clinical Microbiology and Infection 2017 23, 267.e5-267.e7DOI: (10.1016/j.cmi.2016.12.009) Copyright © 2016 The Author(s) Terms and Conditions