The leptin/adiponectin ratio: Potential implications for peritoneal dialysis  D. Teta, M. Maillard, G. Halabi, M. Burnier  Kidney International  Volume 73, Pages S112-S118 (April 2008) DOI: 10.1038/sj.ki.5002611 Copyright © 2008 International Society of Nephrology Terms and Conditions

Figure 1 Interactions between PD fluids, omental adipocytes and adipokines. Normal visceral peritoneal morphology showing a thin mesothelial cell layer lying on a thick sheet of omental adipose tissue. PD fluids are transferred by diffusion through the mesothelium, and therefore may interact with omental adipocytes, which synthesize adipokines. Picture at × 10 original magnification; courtesy of Dr P Furness, Department of Histopathology, Leicester General Hospital, UK. Kidney International 2008 73, S112-S118DOI: (10.1038/sj.ki.5002611) Copyright © 2008 International Society of Nephrology Terms and Conditions

Figure 2 Effect of glucose-based and glucose-free PD fluids on leptin secretion. 3T3-L1 adipocytes in six-well plates were incubated for 48 h in PD4 (glucose 1.36%), ICOD and AA-based PD fluids. M199 was used as the control solution (C). Solutions were diluted 50:50 with M199. Results shown are means±s.e.m. of three independent experiments (pool of 15–28 culture wells for each set of conditions). **P<0.001 vs C. Kidney International 2008 73, S112-S118DOI: (10.1038/sj.ki.5002611) Copyright © 2008 International Society of Nephrology Terms and Conditions

Figure 3 Effect of glucose-based and glucose-free PD fluids on adiponectin secretion. 3T3-L1 adipocytes in six-well plates were incubated for 48 h in PD4 (glucose 1.36%), ICOD, and AA-based PD fluids. M199 was used as the control solution (C). Solutions were diluted 50:50 with M199. Results shown are means±s.e.m. of three independent experiments (pool of 15–28 culture wells for each set of conditions). *P<0.05 vs C. Kidney International 2008 73, S112-S118DOI: (10.1038/sj.ki.5002611) Copyright © 2008 International Society of Nephrology Terms and Conditions

Figure 4 Effect of glucose concentration in dialysates on leptin secretion. 3T3-L1 adipocytes in six-well plates were incubated in laboratory-manufactured PD fluids at various glucose concentrations. Solutions were diluted 50:50 with M199. The final glucose concentration in the test media was as shown. Results shown are those obtained at 48 h of incubation (means±s.e.m. of three independent experiments; pool of 22–26 culture wells for each set of conditions). **P<0.001 vs 2.75 mM glucose. Kidney International 2008 73, S112-S118DOI: (10.1038/sj.ki.5002611) Copyright © 2008 International Society of Nephrology Terms and Conditions

Figure 5 Acute effect of PD4 3.86% on plasma leptin. PD patients (n=5) were subjected to a 6-h dwell with sequentially a high glucose-containing PD fluid (PD4 3.86%), an AA PD fluid, and no PD fluid (C). Patients fasted overnight and during the protocol. Leptin was assayed in plasma after 3 and 6 h (enzyme-linked immunosorbent assay). Mean±s.e.m. % of plasma leptin changes vs initial values are shown. *P<0.05 vs C and AA. Kidney International 2008 73, S112-S118DOI: (10.1038/sj.ki.5002611) Copyright © 2008 International Society of Nephrology Terms and Conditions