Analysis of early changes in the articular cartilage transcriptisome in the rat meniscal tear model of osteoarthritis: pathway comparisons with the rat.

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Analysis of early changes in the articular cartilage transcriptisome in the rat meniscal tear model of osteoarthritis: pathway comparisons with the rat anterior cruciate transection model and with human osteoarthritic cartilage T. Wei, N.H. Kulkarni, Q.Q. Zeng, L.M. Helvering, X. Lin, F. Lawrence, L. Hale, M.G. Chambers, C. Lin, A. Harvey, Y.L. Ma, R.L. Cain, J. Oskins, M.A. Carozza, D.D. Edmondson, T. Hu, R.R. Miles, T.P. Ryan, J.E. Onyia, P.G. Mitchell Osteoarthritis and Cartilage Volume 18, Issue 7, Pages 992-1000 (July 2010) DOI: 10.1016/j.joca.2010.04.012 Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Venn diagram of the number of DE genes identified in the articular cartilage of MT rat model at 3, 7 and 21 days post-MT. The numbers in the parenthesis indicate the number of DE genes identified at three different time points post-surgery in the MT model, while the numbers in the overlapping areas show the number of commonly regulated genes between the three time points. Osteoarthritis and Cartilage 2010 18, 992-1000DOI: (10.1016/j.joca.2010.04.012) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 The number of DE genes commonly identified in the two surgically induced OA rat models (MT and ACLT). Numbers in the parenthesis indicate the number of DE genes identified at different time points in the MT and ACLT models. Numbers in the overlapping areas show the number of commonly regulated genes, with percentages of identities shown in the parenthesis, at 4 weeks in the ACLT and at 3, 7 or 21 days respectively in the MT model. Overlapping genes with fold changes at each time point can be found in the Supplement Table S2. Osteoarthritis and Cartilage 2010 18, 992-1000DOI: (10.1016/j.joca.2010.04.012) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Confirmation of up-regulation of Col5a1 and Col6a2 expression in the MT model by real-time PCR. Confirmation at 1-day time point was not performed due to shortage of RNA. Primers Rn01435869_m1 for Col5a1 and Rn01429506_m1 for Col6a1 were purchased from Applied Biosystems as Assays on Demand™ reagents (Foster City, CA). Specific amplification reactions from the cDNAs were carried out by 2-step real-time PCR and the relative quantities were obtained by generating a standard curve for each gene. The expression levels of genes were normalized to 18S ribosomal RNA used as internal control. All measurements were performed on triplicate samples. Results of real-time PCR were analyzed by Student’s t test and probability (P) values <0.05 were considered statistically significant. Error bars represent the 95% confidence intervals. Osteoarthritis and Cartilage 2010 18, 992-1000DOI: (10.1016/j.joca.2010.04.012) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Up-regulated ECM genes in the articular cartilage of the rat OA models and human OA. MT, meniscus tear; ACLT, anterior cruciate ligament transection; OA, osteoarthritis. Fold changes are calculated as the average expression abundance of a treatment group divided by that of its control group. Osteoarthritis and Cartilage 2010 18, 992-1000DOI: (10.1016/j.joca.2010.04.012) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 DE genes related to programmed cell death defined in Gene Ontology (GO) database. MT, meniscus tear; ACLT, anterior cruciate ligament transection; OA, osteoarthritis. Fold changes are calculated as the average expression abundance of a treatment group divided by that of its control group. Osteoarthritis and Cartilage 2010 18, 992-1000DOI: (10.1016/j.joca.2010.04.012) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 DE genes related to angiogenesis as defined in Gene Ontology (GO) database. MT, meniscus tear; ACLT, anterior cruciate ligament transection; OA, osteoarthritis. Fold change is calculated as the average expression abundance of a treatment group divided by that of its control group. Osteoarthritis and Cartilage 2010 18, 992-1000DOI: (10.1016/j.joca.2010.04.012) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions