A short-term pharmacodynamic model for monitoring aggrecanase activity: injection of monosodium iodoacetate (MIA) in rats and assessment of aggrecan neoepitope.

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Presentation transcript:

A short-term pharmacodynamic model for monitoring aggrecanase activity: injection of monosodium iodoacetate (MIA) in rats and assessment of aggrecan neoepitope release in synovial fluid using novel ELISAs C.A. Swearingen, M.G. Chambers, C. Lin, J. Marimuthu, C.J. Rito, Q.L. Carter, J. Dotzlaf, C. Liu, S. Chandrasekhar, K.L. Duffin, P.G. Mitchell, T.B. Durham, M.R. Wiley, K. Thirunavukkarasu Osteoarthritis and Cartilage Volume 18, Issue 9, Pages 1159-1166 (September 2010) DOI: 10.1016/j.joca.2010.02.019 Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 (a) Schematic of the epitopes recognized in the NITEGE and ARGS ELISAs to measure aggrecanase-generated aggrecan fragments. HABR denotes the motifs present in the G1 and G2 domains of aggrecan. (b) Time-dependent increase in NITEGE neoepitope generation upon ADAMTS-4 digestion of bovine, human and rat aggrecan in the NITEGE ELISA. BAA: bovine articular aggrecan; HAA: human articular aggrecan; RA: rat aggrecan; TS4: ADAMTS-4/aggrecanase-1. (c) Effects of KS on the ability of the ARGS ELISA to measure ADAMTS-4-digested rat or human aggrecan. Rat and human aggrecan were digested overnight with ADAMTS-4 as indicated in Materials and methods. ADAMTS-4-digested rat aggrecan which lacks KS can be measured using this ELISA in the native state without the need for deglycosylation. ADAMTS-4-digested human articular aggrecan must be deglycosylated to remove KS residues to be measured in this ELISA system. Data shown are the mean±standard deviation of representative digests done in duplicate. Osteoarthritis and Cartilage 2010 18, 1159-1166DOI: (10.1016/j.joca.2010.02.019) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Measurement of aggrecanase-cleaved aggrecan fragments in BAC explant cultures treated with IL-1α ± aggrecanase/metalloprotease inhibitors. Conditioned media were collected 72h post treatment and the levels of aggrecanase-cleaved fragments of aggrecan were measured by the NITEGE ELISA (with pooled samples) (a) and s-GAG levels were measured by the DMMB assay (b). Data shown are the mean±standard error of a representative experiment (n=8 explants/treatment). Osteoarthritis and Cartilage 2010 18, 1159-1166DOI: (10.1016/j.joca.2010.02.019) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Time course of aggrecan neoepitope generation in the MIA injection model. MIA was injected intra-articularly into the right knee joint and saline into the left knee joint of male Lewis rats. One group of rats was sacrificed on day 2, 3, 5 or 7, and synovial lavage was collected and analyzed by the NITEGE ELISA (a) and ARGS ELISA (b). n=6 animals/group; s.e.m.: standard error of the mean. Osteoarthritis and Cartilage 2010 18, 1159-1166DOI: (10.1016/j.joca.2010.02.019) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Effect of aggrecanase inhibitors in blocking aggrecanase-dependent generation of aggrecan neoepitopes in the MIA model. n=6 animals/group with MIA (right knee) or saline (left knee) injected intra-articularly. (a) Outline of the MIA model, showing the MIA injection and inhibitor dosing schedules. (b) NITEGE ELISA of synovial lavage obtained from animals treated orally, twice a day, with a broad-spectrum aggrecanase inhibitor (CP-669685) or an MMP inhibitor (RS-130830) at 30mg/kg. (c, d) Analysis of synovial fluid levels of NITEGE (c) and ARGN-containing fragments (d) from animals treated orally, twice a day with a selective aggrecanase inhibitor (compound A) at 3, 10 and 30mg/kg. *Statistical significance when compared to the vehicle treated group (p<0.05). s.e.m.: standard error of the mean. Osteoarthritis and Cartilage 2010 18, 1159-1166DOI: (10.1016/j.joca.2010.02.019) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 NITEGE ELISA (a) and ARGS ELISA (b) of synovial fluid samples obtained from patients at the time of joint replacement surgery. The samples were treated with hyaluronidase to reduce viscosity and then deglycosylated to remove the KS and CS sugars. To determine signal specificity, competition experiments were performed with a 16-mer NITEGE-containing peptide or a 22-mer ARGS-containing peptide. Osteoarthritis and Cartilage 2010 18, 1159-1166DOI: (10.1016/j.joca.2010.02.019) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions