Detection of Trisomy 12 and Rb-Deletion in CD34+ Cells of Patients With B-Cell Chronic Lymphocytic Leukemia by B. Gahn, C. Schäfer, J. Neef, C. Troff,

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Detection of Trisomy 12 and Rb-Deletion in CD34+ Cells of Patients With B-Cell Chronic Lymphocytic Leukemia by B. Gahn, C. Schäfer, J. Neef, C. Troff, M. Feuring-Buske, W. Hiddemann, and B. Wörmann Blood Volume 89(12):4275-4281 June 15, 1997 ©1997 by American Society of Hematology

Representative flow cytometric analysis of patient no. 1. Representative flow cytometric analysis of patient no. 1. Ten thousand cells were aquired in listmode on a FACScan with two gates showing the sorted two different maturational subpopulations: CD34+/38+ cells in fluorescence gate R2 with light scatter gate R1 and CD34+/38− cells in fluorescence gate R3 with light scatter gate R1. B. Gahn et al. Blood 1997;89:4275-4281 ©1997 by American Society of Hematology

The percentage of cells within sorted CD34+/38+ (□) and CD34+/38− () fractions that contain the genetic marker detected by FISH. (▪) The percentage of sort unpurity. The percentage of cells within sorted CD34+/38+ (□) and CD34+/38− () fractions that contain the genetic marker detected by FISH. (▪) The percentage of sort unpurity. B. Gahn et al. Blood 1997;89:4275-4281 ©1997 by American Society of Hematology

Unsorted peripheral blood cells after FISH with a chromosome 12-specific α satellite DNA probe conjugated to spectrum orange. Unsorted peripheral blood cells after FISH with a chromosome 12-specific α satellite DNA probe conjugated to spectrum orange. Three fluorescent hybridization signals indicate trisomy 12; two signals represent diploid cells (left). The right side shows a sorted CD34+/38+ cell hybridized to a spectrum green labeled a chromosome 12-specific α satellite DNA probe. The three hybridization spots indicate trisomy 12 (right). B. Gahn et al. Blood 1997;89:4275-4281 ©1997 by American Society of Hematology

Flow cytometric analysis of bone marrow of a CLL patient stained with CD34 FITC (HPCA-2), CD20 PerCP (L27), and CD5 PE (L17F12). Flow cytometric analysis of bone marrow of a CLL patient stained with CD34 FITC (HPCA-2), CD20 PerCP (L27), and CD5 PE (L17F12). Fifty thousand cells were aquired in listmode on a FACScan. The leukemic cells are depicted blue by simultaneous analysis of CD5 PE and CD20 PerCP, whereas the CD34+ cells appear as green. There is no population detectable coexpressing CD34, CD5, and CD20. B. Gahn et al. Blood 1997;89:4275-4281 ©1997 by American Society of Hematology