MCM3AP‐mediated acetylation of MCM3.

Slides:

Advertisements

Similar presentations

Phenotype Testing and SDS- PAGE Mod2 Day4 Thursday Oct 16th, 2008.

Advertisements

D4476 inhibits the phosphorylation of FOXO1a at Ser322 and Ser325, but not at Thr24 in vitro. D4476 inhibits the phosphorylation of FOXO1a at Ser322 and.

Positioning of the TGF‐β3 and BMP receptor complexes on a membrane surface. Positioning of the TGF‐β3 and BMP receptor complexes on a membrane surface.

Mitochondrial ribosomes remain associated with the inner membrane in the absence of the ribosome‐binding domain of Oxa1 and of Mba1. Mitochondrial ribosomes.

PKA phosphorylates catalytic α‐ and regulatory β‐subunits of AMPK at multiple sites. PKA phosphorylates catalytic α‐ and regulatory β‐subunits of AMPK.

Volume 9, Issue 6, Pages (June 2002)

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

Yeast containing pICC10 (Tir) and pICC267 (CK18) and pTY474S and pICC267 demonstrated an approximately 12.5‐fold increase in β‐galactosidase activity compared.

The RRM of PRT1 is required for its interaction with TIF32/RPG1, HCR1 and NIP1 in vivo. The RRM of PRT1 is required for its interaction with TIF32/RPG1,

Fig. 1. NS1 protein alignment and linear epitope mapping of the 10 antibodies used to run the DENV serotype–specific NS1 rapid tests, pan-DENV NS1 test,

Face‐to‐face kinase domain dimerisation of human Ire1α.

Calcium regulates ERK nuclear association, but not its activation.

Cytochrome c and ATP hydrolysis are required for Apaf‐1 to interact with procaspase‐9. Cytochrome c and ATP hydrolysis are required for Apaf‐1 to interact.

TRF2/RAP1complex mediates C‐NHEJ inhibition at telomeric ends through hindrance at the KU loading and DNA–PK activation steps. TRF2/RAP1complex mediates.

AO domain of LSD1 is responsible for its interaction with Snail1.

The interaction of FAM49B and Rac.

Dimerisation, Auto‐ and substrate‐phosphorylation of CHK2‐KD.

Sequence and comparison of the SDS protein.

Multiple sequence alignment of the BAR domains from Amphiphysins and from the subset of BAR domain family members that were shown to bind to a small GTPase.

Mass spectrometry analysis.

In vitro modification by SUMO‐1 and SUMO‐2 of HDACs and HDAC‐related proteins. 35S‐labelled in vitro translated HDAC1, HDAC3, HDAC6 and MITR were incubated.

Analysis of CLIP‐170 phosphorylation.

Molecular cloning of Xenopus JNK1 and Xenopus MKK7.

MyoD is acetylated on lysines 99 and 102 in myoblasts.

RNA charging levels before and after starvation.

The Protein Import Motor of Mitochondria

E6 Oncoprotein Represses p53-Dependent Gene Activation via Inhibition of Protein Acetylation Independently of Inducing p53 Degradation Mary C. Thomas,

Identification of MOSPD2, a new FFAT motif‐binding protein

The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL.

The FKBP8‐ATG8 interaction is dependent on an intact LIR docking site (LDS)‏ The FKBP8‐ATG8 interaction is dependent on an intact LIR docking site (LDS)

The MSP domain of MOSPD2 binds the FFAT motif

Putative pathogenic variants in KLB identified in congenital hypogonadotropic hypogonadism Putative pathogenic variants in KLB identified in congenital.

MOSPD2 is a putative tail‐anchored protein

Volume 21, Issue 1, Pages (January 2006)

Mechanism of Triton binding to ShHTL7

Volume 7, Issue 9, Pages (September 1997)

GTSF1 interacts with PIWI protein complexes

The MSP domain of MOSPD2 binds the FFAT motif with an affinity in the micromolar range The MSP domain of MOSPD2 binds the FFAT motif with an affinity in.

Volume 96, Issue 3, Pages (February 1999)

Sequence alignment of PHCCEx domains with secondary structure elements of the Tiam2 PHCCEx domain at the top. Sequence alignment of PHCCEx domains with.

Mediator–Nucleosome Interaction

Redox Regulation of Arabidopsis Mitochondrial Citrate Synthase

Heterochromatin Formation in Mammalian Cells

Claudia Schneider, James T. Anderson, David Tollervey Molecular Cell

Specificity of methylation mediated by the COPR5–PRMT5‐containing complex. Specificity of methylation mediated by the COPR5–PRMT5‐containing complex. (A)

Volume 90, Issue 4, Pages (August 1997)

CD44 binding surface of the Tiam2 PHCCEx domain.

Volume 6, Issue 5, Pages (November 2000)

The TAFII250 Subunit of TFIID Has Histone Acetyltransferase Activity

Acetylation status of critical lysine residues in dsRBD affects the binding of DGCR8 to primary miRNA transcripts. Acetylation status of critical lysine.

Analysis of GFP expression in gfp loss-of-function mutants.

Regulation of RECQ4–MCM10 interaction by CDK phosphorylation.

Cloning of a novel gene in the human kidney homologous to rat munc13s: Its potential role in diabetic nephropathy Yong Song, Menachem Ailenberg, Mel.

Palbociclib increases proteasome activity and the clearance of protein aggregates without significant effects on autophagy Palbociclib increases proteasome.

Purification and identification of RECQ4 complex from human cells.

WES identification of heterozygous compound mutations in ALPI

Hartmut Scheel, Kay Hofmann Current Biology

Direct interaction of RECQ4 and MCM10.

Human Pre-mRNA Cleavage Factor Im Is Related to Spliceosomal SR Proteins and Can Be Reconstituted In Vitro from Recombinant Subunits Ursula Rüegsegger,

RIF1 interacts with protein phosphatase 1 isoforms

Volume 12, Issue 4, Pages (February 2002)

The Asf1–histone H3 interaction is reduced in Asf1-185T and 152T mutants. The Asf1–histone H3 interaction is reduced in Asf1-185T and 152T mutants. The.

Alignment of the deduced amino acid sequences of the myosin light chain 2 (MLC2) proteins. Alignment of the deduced amino acid sequences of the myosin.

PP1 preferentially binds nonphosphorylated RV[S/T]F motifs in vitro.

Bacillus subtilis Glutamine Synthetase Controls Gene Expression through a Protein- Protein Interaction with Transcription Factor TnrA Lewis V Wray, Jill.

Volume 5, Issue 6, Pages (June 2000)

Volume 2, Issue 6, Pages (December 1998)

Volume 104, Issue 1, Pages (January 2001)

Volume 1, Issue 4, Pages (March 1998)

Sequence alignment of colicin lysis proteins.

Presentation transcript:

MCM3AP‐mediated acetylation of MCM3. MCM3AP‐mediated acetylation of MCM3. (A) The HAT domain of yeast HAT1 (HAT1), human putative tumor suppressor (FUS2), human Tat interactive protein (60 kDa) (TIP60), and human P/CAF (P/CAF) were compared with MCM3AP. Conserved amino acids between HAT and MCM3AP were indicated by inverted characters. The alignment of MCM3AP was adjusted manually on the basis of classification of amino acid residues: aromatic residues (F, W and Y); negative charged residues (D, E); positive charged residues (H, K, R); aliphatic residues (I, L, and V). (B) MCM3 was incubated with MCM3AP and acetyl CoA or only one of them at 30°C. After incubation the samples were analyzed by SDS–PAGE and blotted to PVDF membrane. (C) After incubation with MCM3AP in the presence or absence of acetyl CoA, the indicated amount of MCM3 was spotted onto nitrocellulose membrane. Acetylated histone H4 peptide was spotted as a standard to evaluate the amount of acetyl moiety. Yoshinori Takei et al. EMBO Rep. 2001;2:119-123 © as stated in the article, figure or figure legend