MyoD is acetylated on lysines 99 and 102 in myoblasts.

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MyoD is acetylated on lysines 99 and 102 in myoblasts. MyoD is acetylated on lysines 99 and 102 in myoblasts. Proteins were extracted from trichostatin A‐treated cells, using either myoblasts (C2C12) or, as a negative control, non‐muscle cells (HeLa). Extracts were immunoprecipitated using MyoD antibody (MyoD) or irrelevant antibodies (Irr.) and then analysed by western blot using purified anti‐acetyl‐K99,102‐MyoD serum (WB: anti‐Ac‐MyoD) or anti‐non‐acetylated‐MyoD (WB: anti‐MyoD) to monitor the total level of MyoD. Recombinant MyoD, either mock acetylated (Non‐Ac) or acetylated (Ac) in vitro, was run on the same gel. K, lysine. Arnaud Duquet et al. EMBO Rep. 2006;7:1140-1146 © as stated in the article, figure or figure legend