Determinants of hepatic effector CD8+ T cell dynamics Alexandre Pierre Benechet, Matteo Iannacone  Journal of Hepatology  Volume 66, Issue 1, Pages 228-233 (January 2017) DOI: 10.1016/j.jhep.2016.07.011 Copyright © 2016 European Association for the Study of the Liver Terms and Conditions

Fig. 1 Liver immunohistochemical micrographs from representative mice that transgenically express HBcAg, all HBV gene products or that have been infected with Ad-HBV-GFP. Top, HBcAg in brown; middle, HBcAg in red; bottom, HBcAg in red and GFP in green. Note that fluorescent hepatocytes expressing HBcAg are also observed after infection with AAV-HBc-GFP (not shown). Percentages of hepatocytes expressing HBV antigens are indicated. Journal of Hepatology 2017 66, 228-233DOI: (10.1016/j.jhep.2016.07.011) Copyright © 2016 European Association for the Study of the Liver Terms and Conditions

Fig. 2 Schematic representation of hepatic effector CD8+ T cell behavior leading to hepatocellular antigen recognition. (1) Effector CD8+ T cells arrest within liver sinusoids by docking onto platelets. (2) They crawl along liver sinusoids, probing hepatocytes for the presence of antigen by extending cellular protrusions through sinusoidal fenestrae. (3) They perform effector function in a diapedesis-independent manner (i.e., while still confined to the intravascular space). Journal of Hepatology 2017 66, 228-233DOI: (10.1016/j.jhep.2016.07.011) Copyright © 2016 European Association for the Study of the Liver Terms and Conditions

Fig. 3 Cell tracks in an intravital multiphoton microscopy movie in the liver of a hepatitis B virus (HBV) replication-competent transgenic mouse. Transferred with antigen-specific (green) or antigen non-specific (red) T cells. Scale bars represent 50μm. Journal of Hepatology 2017 66, 228-233DOI: (10.1016/j.jhep.2016.07.011) Copyright © 2016 European Association for the Study of the Liver Terms and Conditions