Bio 9B: Monday, 11.30.09 Title: Movement Across Cell Membranes – Day 1 Homework: Read pages 182-189 (Section 7-3): Take notes based on the questions on the back of Assignment Sheet and write your answers in your notebook. Do Now: (in your notebook) Which molecule is bigger, starch or glucose? Explain with a diagram in our notebook. Today’s Objectives: Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

Movement Across cell Membranes Moretti and Dickson

Diffusion Across Cell Membranes Designing a Lab: Diffusion Across Cell Membranes Planning the Experiment: Individual: Read Overview Small Groups: Planning the Experiment How do we test for Starch and Glucose? Whole Class Discussion Small Group: Experimental Set-Up Whole Class Review: Model Cell w/ Dialysis Tubing Membrane Starch & Glucose Solution Water Objectives for Class: Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

Diffusion Across Cell Membranes Designing a Lab: Diffusion Across Cell Membranes Class Discussion: Starch How do we test for it? Use Iodine How do we know starch has passed through membrane? Iodine outside the dialysis bag turns from yellow-orange to purplish- brown Glucose Boil it in Benedicts solution or use a glucose test strip How do we know glucose has passed through the membrane? Glucose test strip will change color Objectives for Class: Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

Bio 9B: Tuesday, 12.1.09 Title: Movement Across Cell Membranes – Day 2 Homework: Type the following sections of the Diffusion Lab: Background Info Purpose/Hypothesis Summary of Procedure Do Now: (in your notebook) What is happening to the dye in the water? Based on your reading, what is this called? Today’s Objectives: Design an experiment to test diffusion of starch and glucose across a membrane Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

Diffusion Across Cell Membranes Designing a Lab: Diffusion Across Cell Membranes Review Experimental Set-Up: Part I: Set-up Dialysis Bag Moisten tube and tie one end Add the starch & glucose solution to the tube Tie the top of the tube Rinse w/ tap water to wash excess solution from outside of bag Set-up Beaker Fill a 250 ml beaker ¾ of the way with water Add Iodine to water – mixture should be orange-ish Put dialysis bag into beaker with water and iodine Part II: Wait 30 min to allow for substances to move across the membrane Starch & Glucose Solution Water and Iodine Objectives for Class: Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

Diffusion Across Cell Membranes Designing a Lab: Diffusion Across Cell Membranes Review Experimental Set-Up: Part III: How do you know the following substances have left the bag: Starch? Glucose? What other substances might move across the bag? How will you know? Starch & Glucose Solution Water and Iodine Objectives for Class: Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

New Vocabulary Lipid Bilayer Two layers of lipids that form the outer membrane of a cell. Flexible Strong Do not Dissolve in water zoom out Image to the right shows the lipid bilayer of a cell. Notice how it has other components like protein channels & “pumps” built into it. Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

New Vocabulary Selective Permeability When a cell membrane (or any membrane for that matter) only allows certain materials to pass across the membrane and not others Substance usually pass across based on the size, shape, and charge (+/-/neutral) of the molecules Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

New Vocabulary Concentration Diffusion Osmosis How much “stuff” is in a given amount of liquid Mass of a solute (stuff in the liquid) per a given volume of the total solution i.e.: 12g of salt in 3L of water = 12g/3L = 4g/L Diffusion Movement of molecules from a high concentration (more stuff) to low concentration (less stuff) Osmosis Movement of water through a selectively permeable membrane from a high concentration to low concentration Note: For osmosis to occur, the membrane only allows water to pass across and not the solute Osmosis Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

New Vocabulary Diffusion, Osmosis and Selective Permeability In Action: http://zoology.okstate.edu/zoo_lrc/biol1114/tutorials/Flash/Osmosis_Animation.htm Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

New Seats Jisrael Danika Weston Loic Nick Wilson Emma Chyneree Yikaalo Jeff Graceann Tina Reggie Josh Sophia Rachelle Gaby Christina Andrew Djinnie Mildred Ruth Scarlett Adel Jennifer

Bio 9B: Thursday, 12.3.09 Title: Movement Across Cell Membranes – Day 3 Homework: Type the following sections of the Diffusion Lab: Data/ Results Analysis Conclusion Bring in a printed copy of your background information section to class tomorrow!!!! Do Now: Clear your desks except for lab notebook and the Diffusion Lab Set-up Sheet. Today’s Objectives: Implement an experiment to test diffusion of starch and glucose across a membrane Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell Double Block

Diffusion Across Cell Membranes Implementing the Lab: Diffusion Across Cell Membranes Review Experimental Set-Up: Part I: Preparing Dialysis Bag Soak the dialysis tube in water to soften Tie one end into a knot Fill the tube about half-way w/ starch/ glucose solution Tie the other end of the tube into a knot to seal the bag Rinse w/ tap water to wash excess solution from outside of bag Preparing the Beaker Label your beaker Fill a 140 ml beaker ¾ of the way with water Add 10-15 drops of Iodine to water and mix . Note the color of the water Test water with glucose test strip and note any color change Preparing the Model: Put dialysis bag into beaker with water and iodine solution Part II: Wait 30 min to allow for substances to move across the membrane Part III: Data Collection Diagram the experimental set-up Create a data table to collect data in your notebooks Collect data by recording any color change to the beaker, dialysis bag, and/or glucose test strip. (note: we will finish out notes before you record the data into your table) z Starch & Glucose Solution Water and Iodine Objectives for Class: Implement an experiment to test diffusion of starch and glucose across a membrane

New Vocabulary Facilitated Diffusion When a substance diffuses through a membrane by going through a protein channel Regular diffusion goes through the lipid bilayer Protein Channel Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

New Vocabulary Active Transport When a substance is pumped across a membrane from where there’s a low concentration (less stuff) to where there’s a high concentration (more stuff) Requires a pump (made of protein) and ATP energy! Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

Diffusion Across Cell Membranes Implementing the Lab: Diffusion Across Cell Membranes Review Experimental Set-Up: Part I: Preparing Dialysis Bag Soak the dialysis tube in water to soften Tie one end into a knot Fill the tube about half-way w/ starch/ glucose solution Tie the other end of the tube into a knot to seal the bag Rinse w/ tap water to wash excess solution from outside of bag Preparing the Beaker Label your beaker Fill a 140 ml beaker ¾ of the way with water Add 10-15 drops of Iodine to water and mix . Note the color of the water Test water with glucose test strip and note any color change Preparing the Model: Put dialysis bag into beaker with water and iodine solution Part II: Wait 30 min to allow for substances to move across the membrane Part III: Data Collection Diagram the experimental set-up Create a data table to collect data Collect data by recording any color change to the beaker, dialysis bag, and/or glucose test strip. Starch & Glucose Solution Water and Iodine Objectives for Class: Implement an experiment to test diffusion of starch and glucose across a membrane

Background Information Brainstorm Implementing the Lab: Diffusion Across Cell Membranes Background Information Brainstorm Cell membrane – lipid bilayer Selective Permeability Dialysis tubing (why did we use it, what does it do, characteristics of it) Diffusion Concentration Glucose and starch (what are they, what they do, how big they are, how to test for glucose and starch) Iodine for starch and test strip for glucose, indicators and why they are called that Osmosis (optional) Objectives for Class: Implement an experiment to test diffusion of starch and glucose across a membrane

Bio 9B: Friday, 12.4.09 Title: Movement Across Cell Membranes – Day 4 Homework: Typed Final Draft of the Diffusion Lab Due Monday at the start of Class Do Now: Take out the lab report you are writing. Which molecules moved across the dialysis bag membrane? Why? Today’s Objectives: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

New Vocabulary Diffusion, Osmosis and Selective Permeability In Action: http://zoology.okstate.edu/zoo_lrc/biol1114/tutorials/Flash/Osmosis_Animation.htm Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

Diffusion Across Cell Membranes Wrapping up the Lab: Diffusion Across Cell Membranes Writing Better Background Information Sections Task I: Read the to example Background Information sections from the Denatured Enzyme Lab and grade them according to the rubric. Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

Diffusion Across Cell Membranes Wrapping up the Lab: Diffusion Across Cell Membranes Writing Better Background Information Sections Task II: Create a mind-map to connect the vocabulary and concepts for the Background Information Section. This will help us outline this section for us as well. Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

Background Information Brainstorm Wrapping up the Lab: Diffusion Across Cell Membranes Background Information Brainstorm Cell membrane – lipid bilayer Selective Permeability Dialysis tubing (why did we use it, what does it do, characteristics of it) Diffusion Concentration Glucose and starch (what are they, what they do, how big they are, how to test for glucose and starch) Iodine for starch and test strip for glucose, indicators and why they are called that Osmosis (optional) Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

Diffusion Across Cell Membranes Wrapping up the Lab: Diffusion Across Cell Membranes Writing Better Background Information Sections Task III: Use mind-map/ outline to re-write your background information section more effectively. Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

Diffusion Across Cell Membranes Wrapping up the Lab: Diffusion Across Cell Membranes Data Analysis: Work in your groups to explain what your results mean. If there are color changes, state was this means in terms of what substances are present, but DO NOT explain why the substance is where it is (this should go in the conclusion section) Starch & Glucose Solution Water and Iodine Objectives for Class: Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.