Volume 65, Issue 4, Pages 802-808 (April 2014) Computerized Quantification and Planimetry of Prostatic Capsular Nerves in Relation to Adjacent Prostate Cancer Foci  Johannes Bründl, Sebastian Schneider, Florian Weber, Florian Zeman, Wolf F. Wieland, Roman Ganzer  European Urology  Volume 65, Issue 4, Pages 802-808 (April 2014) DOI: 10.1016/j.eururo.2013.04.043 Copyright © 2013 European Association of Urology Terms and Conditions

Fig. 1 Tumor foci and capsular nerves. (A) Definition of sectors (1–12) and regions for the quantification and computerized planimetry of capsular nerves in a hematoxylin and eosin–stained transverse whole-mount section of the prostate, with tumor marked by the dashed line, tumor stage pT3a, and Gleason score 7; (B) transverse whole-mount section of the prostate, with immunohistochemical nerve staining against S100; (C) 10-fold magnification of the box in Figure 1A, in which the dashed line separates the tumor from the capsule; (D) 10-fold magnification of the box in Figure 1B showing capsular nerves (arrowheads), as well as periprostatic nerves (asterisks), with the prostatic capsule (condensed fibromuscular tissue at the edge of the prostate) marked by dotted lines. V=ventral; VL=ventrolateral; DL=dorsolateral; D=dorsal. European Urology 2014 65, 802-808DOI: (10.1016/j.eururo.2013.04.043) Copyright © 2013 European Association of Urology Terms and Conditions

Fig. 2 Capsular nerves in proximity to adjacent tumor foci. (A) A transverse whole-mount section of the prostate with immunohistochemical staining against S100, tumor marked by dashed lines, tumor stage pT2c, and Gleason score 6; (B) capsule of a tumor-free sector—10-fold magnification of the box in Figure 2A, with condensed fibromuscular tissue at the edge of the prostate marked by dotted lines, capsular nerves shown by arrowheads, and periprostatic nerves shown by asterisks; (C) capsule with adjacent tumor—10-fold magnification of the box in Figure 2A showing capsular nerves (arrowheads) and periprostatic nerves (asterisks), with the prostatic capsule (condensed fibromuscular tissue at the edge of the prostate) marked by dotted lines. European Urology 2014 65, 802-808DOI: (10.1016/j.eururo.2013.04.043) Copyright © 2013 European Association of Urology Terms and Conditions