The value of fast blastocoele re-expansion in the selection of a viable thawed blastocyst for transfer  Yimin Shu, M.D., Ph.D., Jill Watt, Ph.D., Janice Gebhardt, B.S., Jennifer Dasig, M.S., Julie Appling, M.S., Barry Behr, Ph.D.  Fertility and Sterility  Volume 91, Issue 2, Pages 401-406 (February 2009) DOI: 10.1016/j.fertnstert.2007.11.083 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Examples of morphologic changes of frozen blastocysts after thaw and post-thaw culture. (A) Frozen blastocyst immediately after thawing; (B) blastocyst starting to shrink in 0.5 mol/L sucrose; (C) blastocyst shrunk tightly after further dehydration for 10 minutes in 0.2 mol/L sucrose; (D) blastocyst starting to re-expand after 1 hour in vitro culture; (E) blastocyst with >50% blastocoele re-expansion after 2 hours in vitro culture before ET. Original magnification, ×400. Fertility and Sterility 2009 91, 401-406DOI: (10.1016/j.fertnstert.2007.11.083) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Impact of blastomere loss on blastocyst survival (blue bar) and fast blastocoele re-expansion (red bar). Significantly higher rates of blastocyst survival and fast blastocoele re-expansion were obtained for fully intact blastocysts as compared with partially intact blastocysts with different ranges of blastomere loss (P<.01). Fertility and Sterility 2009 91, 401-406DOI: (10.1016/j.fertnstert.2007.11.083) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions