Atypical embryo phenotypes identified by time-lapse microscopy: high prevalence and association with embryo development Kelly Athayde Wirka, M.S., Alice.

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Atypical embryo phenotypes identified by time-lapse microscopy: high prevalence and association with embryo development Kelly Athayde Wirka, M.S., Alice A. Chen, Ph.D., Joe Conaghan, Ph.D., Kristen Ivani, Ph.D., Marina Gvakharia, M.D., Ph.D., Barry Behr, Ph.D., Vaishali Suraj, M.S., Lei Tan, Ph.D., Shehua Shen, M.D. Fertility and Sterility Volume 101, Issue 6, Pages 1637-1648.e5 (June 2014) DOI: 10.1016/j.fertnstert.2014.02.050 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Atypical embryo phenotypes: definition, schematic illustration, and prevalence of abnormal syngamy (AS), abnormal first cytokinesis (A1cyt), abnormal cleavage (AC), and chaotic cleavage (CC). Fertility and Sterility 2014 101, 1637-1648.e5DOI: (10.1016/j.fertnstert.2014.02.050) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Individual screenshots from embryo videos, showing key time points occurring during each dynamic atypical phenotype event, including abnormal syngamy, abnormal first cytokinesis, abnormal cleavage, and chaotic cleavage. See Supplemental Videos 1–8 (available online at www.fertstert.org) for full-length embryo videos, which capture embryo development every 5 minutes (post fertilization status assessment to day 3 for abnormal first cytokinesis [A1cyt], abnormal cleavage [AC] and chaotic cleavage [CC]) using low-power, darkfield illumination. Labels on each screenshot denote the frame number from the full-length video. Fertility and Sterility 2014 101, 1637-1648.e5DOI: (10.1016/j.fertnstert.2014.02.050) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Prevalence of embryos with one or more phenotypes and screenshots of an embryo exhibiting abnormal first cytokinesis (A1cyt) and abnormal cleavage (AC). See Supplemental Video 9 (available online at www.fertstert.org) for full-length embryo videos which capture embryo development every 5 minutes (postfertilization status assessment to day 3) with the use of low-power darkfield illumination. Labels on each screenshot denote the frame number from the full-length video. Fertility and Sterility 2014 101, 1637-1648.e5DOI: (10.1016/j.fertnstert.2014.02.050) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions