Aminopeptidase activity in human nasal mucosa

Slides:

Advertisements

Similar presentations

Food allergy to honey: Pollen or bee products?

Advertisements

Anaphylaxis induced by horsefly bites: Identification of a 69 kd IgE-binding salivary gland protein from Chrysops spp. (Diptera Tabanidae) by western.

Identification of patatin as a novel allergen for children with positive skin prick test responses to raw potato Ulla Seppälä, MSca,b, Harri Alenius,

Identification of common allergenic structures in mugwort and ragweed pollen Reinhold Hirschwehr, MDa, Claudia Heppner, MSca, Susanne Spitzauer, MDb,

Eosinophil cationic protein and histamine after intestinal challenge in patients with cow's milk intolerance Ulf Bengtsson, MD, PhDa, Tina W. Knutson,

Wheat α-amylase inhibitor: A second route of allergic sensitization

Christopher L. Kepley, PhD, Bridget S. Wilson, PhD, Janet M

Food allergy to honey: Pollen or bee products?

Gloria García-Casado, PhD, Jesús F

Damage of the pharyngeal mucosa and hyperresponsiveness of airway in sinusitis Giovanni Rolla, MDa, Paola Colagrande, MDa, Ermanno Scappaticci, MDb, Flavia.

Hong Wei Chu, MDa, Monica Kraft, MDa, James E. Krause, PhDb, Michael D

Effects of topical glucocorticoids on in vitro lactoferrin glandular secretion: Comparison between human upper and lower airways Jordi Roca-Ferrer, MSa,

Sabine Fischer, MSc,a, Monika Grote, PhD,b, B. Fahlbusch, PhD,c, W. D

Allergen binding to specific IgE in the nasal mucosa of allergic patients Alex KleinJan, BSca, Tom Godthelp, MDa, Albert van Toornenenbergen, PhDb, Wytske.

Araceli Díaz-Perales, PhDa, Ana I

Practice Notes from the AAAI

Mechanism of neutrophil recruitment induced by IL-8 in chronic sinusitis Hideaki Suzuki, MDa, Yuichi Takahashi, MDb, Hideya Wataya, MDa,c, Katsuhisa Ikeda,

Requirements for allergen-induced airway inflammation and hyperreactivity in CD4- deficient and CD4-sufficient HLA-DQ transgenic mice Svetlana P. Chapoval,

Allergy to eggs from duck and goose without sensitization to hen egg proteins Belén Añíbarro, MD, F.Javier Seoane, MD, Concepción Vila, MD, Manuel Lombardero,

Severe oral allergy syndrome and anaphylactic reactions caused by a Bet v 1– related PR-10 protein in soybean, SAM22 Jörg Kleine-Tebbe, MDa, Andrea Wangorsch,

Serum levels of eosinophil cationic protein in allergic diseases and natural allergen exposure Margherita Tomassini, MDa, Laura Magrini, MDa, Guido De.

Efficacy of local nasal immunotherapy for Dp2-induced airway inflammation in mice: Using Dp2 peptide and fungal immunomodulatory peptide Yi-Hsia Liu,

Molecular characterization of dog albumin as a cross-reactive allergen

Major cat and dog allergens share IgE epitopes

Nobuhisa Terada, MDa, Akiyoshi Konno, MDb, Kiyoshi Togawa, MDa

David D. Tieu, MD, Anju T. Peters, MD, Roderick T

Stem cell factor in nasal polyposis and allergic rhinitis: Increased expression by structural cells is suppressed by in vivo topical corticosteroids

Anaphylaxis to beans Journal of Allergy and Clinical Immunology

Allergic rhinoconjunctivitis caused by Harmonia axyridis (Asian lady beetle, Japanese lady beetle, or lady bug) John A. Yarbrough, MDa, Jack L. Armstrong,

Increased sensitivity to bradykinin among African Americans

Expression and localization of histamine H2 receptor messenger RNA in human nasal mucosa Noriko Hirata, MDa, Kazuhiko Takeuchi, MDa, Kotaro Ukai, MDa,

News & Notes Journal of Allergy and Clinical Immunology

Correlation between alcohol-induced asthma and acetaldehyde dehydrogenase-2 genotype Atsuko Takao, MDa, Terufumi Shimoda, MDa, Shigeru Kohno, MDa, Sadahiro.

Cross-reactivity between Cupressus arizonica and Cupressus sempervirens pollen extracts Bianca Barletta, BSc, Claudia Afferni, BSc, Raffaella Tinghino,

Basophils and eosinophils in allergic rhinitis

Stem cell factor in nasal polyposis and allergic rhinitis: Increased expression by structural cells is suppressed by in vivo topical corticosteroids

Class I chitinases as potential panallergens involved in the latex-fruit syndrome Carlos Blanco, PhDa, Araceli Diaz-Perales, BSb, Carmen Collada, PhDc,

Identification of patatin as a novel allergen for children with positive skin prick test responses to raw potato Ulla Seppälä, MSca,b, Harri Alenius,

Classification of anti-FcϵRI and anti-IgE autoantibodies in chronic idiopathic urticaria and correlation with disease severity Ruth A. Sabroe, MDa, Edda.

Corticosteroid-induced apoptosis in mouse airway epithelium: Effect in normal airways and after allergen-induced airway inflammation Delbert R. Dorscheid,

Genetic susceptibility to food allergy is linked to differential TH2-TH1 responses in C3H/HeJ and BALB/c mice Vivian Morafo, PhD*, Kamal Srivastava,

Fatal outcome of anaphylaxis to camomile-containing enema during labor: A case study Erika Jensen-Jarolim, MDa, Norbert Reider, MDb, Ruth Fritsch, MDa,

Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of patients with hay fever after local allergen provocation Alex.

Occupational allergy caused by urticating hair of Brazilian spider

Increased urinary excretion of the prostaglandin D2 metabolite 9α,11β-prostaglandin F2 after aspirin challenge supports mast cell activation in aspirin-induced.

Relation of epidermal growth factor receptor expression to goblet cell hyperplasia in nasal polyps Pierre-Regis Burgel, MDa, Estelle Escudier, MDb, Andre.

Beer anaphylaxis Journal of Allergy and Clinical Immunology

Potential role of interleukin-1 in allergen-induced late asthmatic reactions in guinea pigs: Suppressive effect of interleukin-1 receptor antagonist on.

Sensitization to the major allergen of Brazil nut is correlated with the clinical expression of allergy Elide A. Pastorello, MDa, Laura Farioli, BScb,

Loratadine reduces allergen-induced mucosal output of α2-macroglobulin and tryptase in allergic rhinitis Lennart Greiff, MD, PhDa, Carl G.A. Persson,

Ligation of IgE receptors causes an anaphylactic response and neutrophil infiltration but does not induce eosinophilic inflammation in mice Masayuki.

Chemokines in seasonal allergic rhinitis

Subpollen particles: Carriers of allergenic proteins and oxidases

Evidence that enhanced nasal reactivity to bradykinin in patients with symptomatic allergy is mediated by neural reflexes Margerita M. Riccio, PhD, David.

Intranasal administration of eotaxin increases nasal eosinophils and nitric oxide in patients with allergic rhinitis Toyoyuki Hanazawa, MD, PhD, Julio.

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Changes in sputum counts and airway hyperresponsiveness after budesonide: Monitoring anti-inflammatory response on the basis of surrogate markers of airway.

Monocyte chemotactic proteins in allergen-induced inflammation in the nasal mucosa: Effect of topical corticosteroids Pota Christodoulopoulos, BSca,

Local expression of ϵ germline gene transcripts and RNA for the ϵ heavy chain of IgE in the bronchial mucosa in atopic and nonatopic asthma Sun Ying,

Abnormalities of cell and mediator levels in bronchoalveolar lavage fluid of patients with mild asthma Sally E. Wenzel, MD Journal of Allergy and Clinical.

Identification of IL-16 as the lymphocyte chemotactic activity in the bronchoalveolar lavage fluid of histamine-challenged asthmatic patients Margaret.

IL-4 upregulates FcϵRI α-chain messenger RNA in eosinophils

IL-4– and IL-5–positive T lymphocytes, eosinophils, and mast cells in allergen-induced late-phase cutaneous reactions in atopic subjects Luis T. Barata,

Allergenicity of goat’s milk in children with cow’s milk allergy

Philippe A. Eigenmann, MD,a, A. Wesley Burks, MD,b, Gary A

Production of a recombinant imported fire ant venom allergen, Sol i 2, in native and immunoreactive form Margit Schmidt, PhDa, b, Thomas J. McConnell,

Histamine release in intact human skin by monocyte chemoattractant factor-1, RANTES, macrophage inflammatory protein-1α, stem cell factor, anti-IgE, and.

The effects of gastric digestion on codfish allergenicity

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Molecular cloning of a major Alternaria alternata allergen, rAlt a 2

Presentation transcript:

Aminopeptidase activity in human nasal mucosa Kimihiro Ohkubo, MDa, James N. Baraniuk, MDb, Robert Hohman, PhDc, Marco Merida, MDd, Louis B. Hersh, MDe, Michael A. Kaliner, MDf Journal of Allergy and Clinical Immunology Volume 102, Issue 5, Pages 741-750 (November 1998) DOI: 10.1016/S0091-6749(98)70013-2 Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 1 Inhibition of leucine-enkephalin–degrading aminopeptidase (leu-AP) by bestatin (open circles ) or puromycin (closed circles ) in human nasal mucosal S2 and P2 fractions. Journal of Allergy and Clinical Immunology 1998 102, 741-750DOI: (10.1016/S0091-6749(98)70013-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 2 Substrate competition study in human nasal mucosal extracts. A , Leucine-enkephalin–degrading aminopeptidase (leu-Ap) activities in S2 fractions (open circles ) were inhibited and shifted right by addition of alanine-p -nitroanilide (ala-NA) (filled circles ). B, Leu-Ap activities in P2 fractions (open circles ) were inhibited and shifted right by adding ala-NA (filled circles ). C, Alanine aminopeptidase (ala-Ap) activity of S2 fractions (open circles ) was inhibited and shifted right by adding leucine enkephalin (leu-enk, filled circles) . D, Ala-Ap activities in P2 fractions (open circles ) were inhibited and shifted right by adding leu-enk (filled circles ). Journal of Allergy and Clinical Immunology 1998 102, 741-750DOI: (10.1016/S0091-6749(98)70013-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 3 Native-PAGE and enzyme blot of human nasal mucosa extracts. Lane 1 , Concentrated S2 fraction; Lane 2 , concentrated P2 fraction; Lane 3 , S2 fraction; Lane 4 , P2 fraction. A , Native-PAGE gel was stained by Coomassie blue. B , Alanine-aminopeptidase activity zymogram. Journal of Allergy and Clinical Immunology 1998 102, 741-750DOI: (10.1016/S0091-6749(98)70013-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 4 Puromycin-sensitive aminopeptidase (PS-Ap)-immunoreactive material in human nasal mucosa. A, PS-Ap–immunoreactive material (intensely stained black material) was present in glycocalyx and epithelial cells (e) . Goblet cells did not stain for PS-Ap. Endothelial surfaces of small veins (arrow heads *) stained slightly. B, Nonimmune serum negative control. C, PS-Ap immunoreactivity was noted in inner lumen of gland ducts and glandular cells (g) . D, Negative control. Nuclear fast red counterstain. Bars represent 200 μm. Journal of Allergy and Clinical Immunology 1998 102, 741-750DOI: (10.1016/S0091-6749(98)70013-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 5 Puromycin-sensitive aminopeptidase (PS-Ap)-immunoreactive material in human nasal mucosa. A, PS-Ap–immunoreactive material (intensely stained black material) was present in glandular serous cells (s) but not in mucous cell vacuoles (m) . Endothelium of veins (v) was also stained. B, Nonimmune serum negative control. Nuclear fast red counterstain. Bar represents 50 μm. Journal of Allergy and Clinical Immunology 1998 102, 741-750DOI: (10.1016/S0091-6749(98)70013-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 6 Zymogram of aminopeptidase M activity in human nasal mucosa. A, l- alanine-4-methoxy-2-naphthylamide–cleaving aminopeptidase was localized in epithelium (e) and submucosal glands (g) . B, Inhibition control. Adjacent sections preincubated with 1 mmol/L puromycin showed no aminopeptidase activity. C, Aminopeptidase activity was most concentrated in central region of glandular acini. D, Inhibition control for C . Bars represent 100 μm. Journal of Allergy and Clinical Immunology 1998 102, 741-750DOI: (10.1016/S0091-6749(98)70013-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 7 Methacholine and histamine-induced nasal secretions. Correlations between nasal lavage fluid concentrations of leucine-aminopeptidase (leu-Ap) activity and total protein (TP) (A, D ), lactoferrin (LF) (B, E ), and albumin (ALB) (C, F ) are shown after methacholine (A, B, C ) and histamine (D, E, F ) provocations. Coefficients of variation (r ) were determined by linear regression. Journal of Allergy and Clinical Immunology 1998 102, 741-750DOI: (10.1016/S0091-6749(98)70013-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 8 Bestatin-inhibitable leucine-enkephalin–degrading aminopeptidase (leu-Ap) activity in nasal lavage fluids after antigen challenge (mean ± SEM). Atopic subjects (n = 8) received serial saline nasal lavages for 50 minutes, then were challenged topically with allergen at 60 minutes. Lavages were then collected at 10-minute intervals between 70 and 120 minutes and at 20-minute intervals between 140 and 180 minutes. Journal of Allergy and Clinical Immunology 1998 102, 741-750DOI: (10.1016/S0091-6749(98)70013-2) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 9 Allergen-induced nasal secretions. Data and coefficients of variation (r ) determined by linear regression are shown for secretion of leucine-enkephalin–degrading aminopeptidase (leu-Ap ) and A , total protein (TP ); B , lactoferrin (LF ); and C , IgG (IGG ) concentrations. Journal of Allergy and Clinical Immunology 1998 102, 741-750DOI: (10.1016/S0091-6749(98)70013-2) Copyright © 1998 Mosby, Inc. Terms and Conditions