Sperm chromatin damage impairs human fertility‡ Marcello Spanò, Ph.D., Jens P Bonde, Ph.D., Henrik I Hjøllund, Ph.D., Henrik A Kolstad, Ph.D., Eugenia Cordelli, B.Sc., Giorgio Leter  Fertility and Sterility  Volume 73, Issue 1, Pages 43-50 (January 2000) DOI: 10.1016/S0015-0282(99)00462-8

Figure 1 Representative green (FL1-H) vs. red (FL3-H) fluorescence bivariate cytogram (A) together with the corresponding αT histogram (B) of one sample measured by the FCM SCSA. The fraction of cells with abnormal chromatin (COMP αT) is boxed off in the cytogram and in the corresponding frequency histogram. Spanò. Sperm chromatin damage. Fertil Steril 2000. Fertility and Sterility 2000 73, 43-50DOI: (10.1016/S0015-0282(99)00462-8)

Figure 2 The unadjusted relation between fecundability and SCSA parameters. (A), × αT (1,301 cycles). (B), COMP αT (1,301 cycles). (C), × green (1,301 cycles). (D), COMP αT (first 215 cycles). The relationships are graphically represented by smoothing linear regression for binomially distributed data. The circles indicate the observed frequency of pregnancy in decentils of the SCSA parameters. The dotted lines are the 95% CI. Spanò. Sperm chromatin damage. Fertil Steril 2000. Fertility and Sterility 2000 73, 43-50DOI: (10.1016/S0015-0282(99)00462-8)