Bordetella parapertussis isolates not expressing pertactin circulating in France  V. Bouchez, D. Brun, G. Dore, E. Njamkepo, N. Guiso  Clinical Microbiology and Infection  Volume 17, Issue 5, Pages 675-682 (May 2011) DOI: 10.1111/j.1469-0691.2010.03303.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Genomic analysis of Bordetella parapertussis isolates: chromosomal DNA profiles obtained after digestion with XbaI; the name of the isolates; the country; the year of collection. Clinical Microbiology and Infection 2011 17, 675-682DOI: (10.1111/j.1469-0691.2010.03303.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Invasive properties of different Bordetella parapertussis isolates. Invasion of human tracheal epithelial (HTE) cells was performed with 12822, BPP1 (PRN‐positive), FR3728 (PRN‐negative), FR3717 (PRN‐negative), FR3743 (PRN‐negative), FR3772 (PRN‐negative), FR3286 (PRN‐positive) and FR0388 (PRN‐positive). Each isolate was added to one well in a 24‐well tissue culture plate containing at least 105 cells/mL, such that the bacterium/cell ratio was 100 : 1. The invasion of HTE cells was assayed as described in Materials and Methods. Invasion by each isolate is reported relative to that of the B. parapertussis reference strain 12822. Values are the means and standard deviations of at least three experiments. Clinical Microbiology and Infection 2011 17, 675-682DOI: (10.1111/j.1469-0691.2010.03303.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Bacterial lung colonization observed in mice after intranasal challenge with Bordetella parapertussis isolates. The reference strain 12822 and the isolates BPP1 (pertactin (PRN)‐positive) and FR3728 (PRN‐negative) were used. Groups of 4‐week‐old Swiss mice (n = 4) were challenged with 50 μL of bacterial suspension containing 5 × 106 CFU of B. parapertussis. Bacterial lung colonization was measured at the indicated time points after challenge, and the results are expressed as the weighted mean CFU per lung (log10‐transformed). The plots show the geometric means for four mice per time point. Clinical Microbiology and Infection 2011 17, 675-682DOI: (10.1111/j.1469-0691.2010.03303.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions