High efficiency of gene deletion in all tested genetic backgrounds of A. fumigatus. High efficiency of gene deletion in all tested genetic backgrounds.

Slides:

Advertisements

Similar presentations

PCR genotype analysis to determine RNP-mediated knockout efficiency in C. lusitaniae. PCR genotype analysis to determine RNP-mediated knockout efficiency.

Advertisements

Expansion of Interstitial Telomeric Sequences in Yeast

CRISPR/Cas targeting of RFP in C. albicans.

cpDNAs with large deletions accumulate in cptk1 mutants.

MFS1 expression in IPO323 MFS1 replacement mutants.

Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ.

Reversing the TERT promoter mutation to WT reverses the active chromatin marks and alters long-range chromatin interactions. Reversing the TERT promoter.

Growth characteristics of strain 22A and its MDH mutant derivatives.

Yeast Origins Establish a Strand Bias for Replicational Mutagenesis

5-ALA increased porphyrin production, which was further enhanced by vitamin B12 supplementation in acne-associated type IA-2 strains. 5-ALA increased porphyrin.

Conditions that countered intracellular alkalization increased M

Phylogeny of Shiga toxin-encoding phage from SDi/SJo S. sonnei isolates. Phylogeny of Shiga toxin-encoding phage from SDi/SJo S. sonnei isolates. (A) progressiveMauve.

Isolation and phylogenetic characterization of ISS strains.

Synthetic perturbation of DE and DV genes.

Unified Solo vectors for mutagenesis in C. albicans.

ERMES impacts on the exposure of pathogen-associated molecular patterns during hyphal growth. ERMES impacts on the exposure of pathogen-associated molecular.

Individual genes exhibit distinct stage-specific expression among female gametocytes. Individual genes exhibit distinct stage-specific expression among.

Quantification of drug cross-resistance between mutants selected for I-BET151 resistance and F0002 resistance. Quantification of drug cross-resistance.

Growth of L. monocytogenes strains AL4E, AT3E, and 10403S, derivative cured strain AT3Epc, and conjugation strains 10403SpclpL and 10403SpPL2 displayed.

Individual genes exhibit distinct female-enriched expression patterns.

Bar plot representation of the transcriptomic changes in Δsaci_ptp and Δsaci_pp2a. Bar plot representation of the transcriptomic changes in Δsaci_ptp and.

RFP CRISPR mutagenesis as a function of sgRNA delivery.

Conserved Histone Variant H2A

Effect of small TbTim knockdown on cell growth.

Effect of dinB gene deletion on the frequency of spontaneously arisen TetR mutants in alkA tag mutS background. Effect of dinB gene deletion on the frequency.

Effect of dinB gene deletion on the frequency of spontaneously arisen TetR mutants in different genetic backgrounds. Effect of dinB gene deletion on the.

Δcarp mutants have a growth phenotype in vitro and in vivo.

Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant with Triton X-100. Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant.

Antibiofilm effects of zinc pyrithione and silver sulfadiazine individually and in combination against S. aureus, A. baumannii, and P. aeruginosa. Antibiofilm.

Southern blot analysis of ΔpksP mutant generated in the Af293 background. Southern blot analysis of ΔpksP mutant generated in the Af293 background. (A)

Efficient CRISPR mutagenesis in C. glabrata.

Antibody reactivity against HA1 proteins after vaccination.

Phenotypic consequences of the most common rpoB mutants.

Impact of the position of the substituted nucleotide in the repair template on repair rates. Impact of the position of the substituted nucleotide in the.

Distribution of deletions and insertions in the ade6-(GACC)7ΔT repeat.

Cell density and secreted enzymes contribute to aggregate invasive growth. Cell density and secreted enzymes contribute to aggregate invasive growth. (A)

GATC sites where methylation changes over time correspond to a change in gene expression in a dam mutant. GATC sites where methylation changes over time.

Change in fluorescence of C. neoformans upon addition of DM262.

Proteus mirabilis strain HI4320 (WT) and isogenic mutants for dsdA, dsdC, or dsdX were cultured in LB broth overnight and diluted 1:100 into fresh LB (A),

Strategy for marker recycling through CRISPR-Cas9-induced marker excision. Strategy for marker recycling through CRISPR-Cas9-induced marker excision. Consider.

Editing of ADE2 in C. metapsilosis.

Expression of all the GAP genes is under control of the Csy1 sensor and regulated by nitrogen sources. Expression of all the GAP genes is under control.

Not all uropathogens can utilize d-serine as a sole source of carbon or nitrogen. Not all uropathogens can utilize d-serine as a sole source of carbon.

SusG LES is required for efficient packing into OMVs

SAM transport by Gap4 in S. cerevisiae cells.

Phenotypic characterization of low-Cu-responsive transcription factor Mac1 in A. nidulans. Phenotypic characterization of low-Cu-responsive transcription.

Using CRISPRi to knock down acm2 expression in L. plantarum WCFS1.

Molecular characterization of the key motifs of AnMac1.

Bacterial composition of olive fermentations is affected by microbial inoculation. Bacterial composition of olive fermentations is affected by microbial.

KEGG cluster of DEGs of S

The pCT-tRNA plasmid system for gene editing in C. tropicalis.

MMEJ plays a dominant role in double-strand DNA break repair in L

CT production profiles of single-SNP strains and double-, triple-, and quadruple-SNP combination strains. CT production profiles of single-SNP strains.

Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2. Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2. (A)

dCas9-mediated repression of transcription.

Genotype analysis of transient CRISPR system transformants.

Shannon rarefaction analysis.

Small-molecule inhibitor reduced porphyrin production in P

Transient CRISPR-Cas9 system.

PHs of olive brines. pHs of olive brines. The average ± standard deviation (SD) of two independent replicates at each time point is shown. Acetic acid.

Dose-dependent growth inhibition assays of fluconazole and DM262.

Production of ade2Δ/ade2Δ mutants by the Candida CRISPR system.

Expression and functional characterization of Cu transporters CtrA2 and CtrC in A. nidulans. Expression and functional characterization of Cu transporters.

WLBU2 disrupts P. aeruginosa biofilms during RSV coinfection of bronchial epithelial cells. WLBU2 disrupts P. aeruginosa biofilms during RSV coinfection.

Impact of the SOS activities on E. coli mutation rates.

GBM cells infected in vitro with HCMV divide less often than uninfected cells do. GBM cells infected in vitro with HCMV divide less often than uninfected.

GBM 112 and GBM 144 cells infected in vitro with HCMV resist growth inhibition by temozolomide. GBM 112 and GBM 144 cells infected in vitro with HCMV resist.

Expression levels of additional giardia genes in elutriation fractions

Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen. Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen.

Knock-in of the rpl42-P56Q mutation using the split-ura4 system.

Presentation transcript:

High efficiency of gene deletion in all tested genetic backgrounds of A. fumigatus. High efficiency of gene deletion in all tested genetic backgrounds of A. fumigatus. In vitro-assembled Cas9 RNPs coupled with microhomology-mediated integration of the HygR cassette were tested in ΔakuB (A), Af293 (B), and DI15-102 (C) strains. (Above) Representative transformation plates are shown for each strain using 2 µg of the HygR repair template that is flanked by 35-bp microhomology arms. (Below) The assessment of pksP deletion efficiency across different strains is plotted as the number of ΔpksP mutants out of the total number of transformation colonies. Deletion efficiencies were assessed based on the color of conidia. The ΔpksP mutant produces white colonies, while ectopic integrations result in green colonies. Deletion efficiencies represent the average from at least three independent transformations. Error bars represent the standard deviation calculated for each combination of strain, the size of HygR microhomology arms, and concentration of the HygR repair template for all experimental replicates. Qusai Al Abdallah et al. mSphere 2017; doi:10.1128/mSphere.00446-17