Allergen-dependent solubilization of IL-13 receptor α2 reveals a novel mechanism to regulate allergy Michael O. Daines, MD, Weiguo Chen, PhD, Yasuhiro.

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Presentation transcript:

Allergen-dependent solubilization of IL-13 receptor α2 reveals a novel mechanism to regulate allergy Michael O. Daines, MD, Weiguo Chen, PhD, Yasuhiro Tabata, MD, PhD, Bradley A. Walker, Aaron M. Gibson, AS, J. Alexander Masino, PhD, Manoj R. Warrier, MD, Cori L. Daines, MD, Sally E. Wenzel, MD, Gurjit K. Khurana Hershey, MD, PhD Journal of Allergy and Clinical Immunology Volume 119, Issue 2, Pages 375-383 (February 2007) DOI: 10.1016/j.jaci.2006.09.039 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 HDM treatment results in solubilization of IL-13Rα2. A, U937 (U) and FLAG–IL-13Rα2 transfected U937 cells (T) were treated with HDM. Soluble IL-13Rα2 was quantified. B, FLAG–IL-13Rα2 transfected U937 cells were treated with the indicated allergen extracts. Soluble IL-13Rα2 was quantified. C, Media from FLAG–IL-13Rα2 transfected cells treated with HDM for minutes as indicated. D, FLAG–IL-13Rα2 transfectants were treated with HDM, alone or with protease inhibitors (+I). Soluble IL-13Rα2 was quantified by ELISA. Significant differences noted. NT, No treatment. ∗Significantly different from untreated, P < .05. Journal of Allergy and Clinical Immunology 2007 119, 375-383DOI: (10.1016/j.jaci.2006.09.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Direct cleavage of FLAG–IL-13Rα;2 by allergens. GST–FLAG–IL-13Rα2 was incubated with the indicated allergens or media for 1 hour, analyzed by PAGE, and detected by Western blot using anti-FLAG (A and C) or anti–IL-13Rα2 antibodies (B). Western blot is representative of 3 experiments. NT, No treatment. Journal of Allergy and Clinical Immunology 2007 119, 375-383DOI: (10.1016/j.jaci.2006.09.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Effect of HDM treatment on surface IL-13Rα2. A, Surface IL-13Rα2 expression was determined after exposure to HDM (circles) or trypsin (squares) for the indicated times. B, Total IL-13Rα2 in cellular lysates was determined after HDM (gray bars) or trypsin (white bars) treatment (∗P < .05 compared with transfected untreated lysate). Means ± SDs of 4 experiments shown. MCF, Mean channel fluorescence. Journal of Allergy and Clinical Immunology 2007 119, 375-383DOI: (10.1016/j.jaci.2006.09.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Confocal microscopy reveals maintenance of steady-state surface level of IL-13Rα2. FLAG–IL-13Rα2 transfectants were labeled with anti-FLAG antibodies at 4°C, washed, and incubated at 37°C for the indicated times. Remaining surface-bound FLAG antibodies were detected by confocal microscopy. After the 30-minute incubation, some cells were restained for surface FLAG–IL-13Rα2. Representative images from 3 independent experiments shown. Journal of Allergy and Clinical Immunology 2007 119, 375-383DOI: (10.1016/j.jaci.2006.09.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 HDM treatment and A fumigatis treatment have opposite effects on IL-13–dependent Stat6 activation. A, Cells were incubated with or without HDM and stimulated with IL-13 or IL-4 for 20 minutes. Stat6 activation by EMSA. B and C, Means ± SEMs of densitometry of 3 experiments. ∗P = .02. D, FLAG–IL-13Rα2 transfected cells were incubated with or without A fumigatus and stimulated with cytokine. Stat6 activation by EMSA. AF, Aspergillus fumigatus. Journal of Allergy and Clinical Immunology 2007 119, 375-383DOI: (10.1016/j.jaci.2006.09.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Soluble IL-13Rα2 in vivo. C57BL/6 mice were treated once with intratracheal HDM or PBS, and levels of soluble IL-13Rα2 (sIL-13Rα2) were determined in (A) BAL fluid and (B) serum by ELISA. Means ± SDs shown (N = 5 mice in each group). C, sIL-13Rα2 in BAL fluid obtained from subjects with asthma (N = 11) or control subjects (N = 23) was quantified. Means ± SDs shown. Journal of Allergy and Clinical Immunology 2007 119, 375-383DOI: (10.1016/j.jaci.2006.09.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 7 Schematic representation of effects of HDM and mold allergen exposure on IL-13 receptors and signaling. Journal of Allergy and Clinical Immunology 2007 119, 375-383DOI: (10.1016/j.jaci.2006.09.039) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions