Rapid Simultaneous Screening of Factor V Leiden and G20210A Prothrombin Variant by Multiplex Polymerase Chain Reaction on Whole Blood by Encarnación Gómez, Sonja C.P.A.M. van der Poel, Joop H. Jansen, Bert A. van der Reijden, and Bob Löwenberg Blood Volume 91(6):2208-2209 March 15, 1998 ©1998 by American Society of Hematology

Multiplex detection of wild-type and mutated FII (G20210A) and FV (G1691A) using whole blood (2 μL) as the DNA source in PCR. Lanes 1 through 3, undigested multiplex PCR products using the described conditions for FII (lane 1 [32 cycles] and 3 [40 cycles]) ... Multiplex detection of wild-type and mutated FII (G20210A) and FV (G1691A) using whole blood (2 μL) as the DNA source in PCR. Lanes 1 through 3, undigested multiplex PCR products using the described conditions for FII (lane 1 [32 cycles] and 3 [40 cycles]) and for FV (lane 2). Lanes 4 through 9, undigested and Mnl I and HindIII double-digested PCR product electrophoresis of a patient double heterozygote for FII and FV; undigested and digested factor FV fragment (lanes 4 and 5, respectively); undigested and digested FII fragment (lanes 6 and 7, respectively); undigested and digested FII and FV fragments obtained after multiplex PCR (lanes 8 and 9, respectively). Lanes 10 through 13, double-digested products obtained after multiplex PCR from a normal individual (lane 10), an FII and FV Leiden double heterozygote (lane 11), an FII heterozygote (lane 12), and an FV Leiden heterozygote (lane 13). Products were separated on a 2.5% agarose gel (per lane, 20 μL of the digestion mix is loaded). Marker is a 100-bp size marker (GIBCO BRL, Grand Island, NY). Relevant fragment sizes in basepairs are indicated. Encarnación Gómez et al. Blood 1998;91:2208-2209 ©1998 by American Society of Hematology