Detection of Microsatellite Instability in Colorectal Cancer Using an Alternative Multiplex Assay of Quasi-Monomorphic Mononucleotide Markers Vanessa.

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Detection of Microsatellite Instability in Colorectal Cancer Using an Alternative Multiplex Assay of Quasi-Monomorphic Mononucleotide Markers Vanessa Deschoolmeester, Marc Baay, Wim Wuyts, Eric Van Marck, Nancy Van Damme, Peter Vermeulen, Krzysztof Lukaszuk, Filip Lardon, Jan B. Vermorken The Journal of Molecular Diagnostics Volume 10, Issue 2, Pages 154-159 (March 2008) DOI: 10.2353/jmoldx.2008.070087 Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Examples of MSS (A) and MSI-H (B and C) profiles generated with the quasi-monomorphic mononucleotide multiplex PCR assay. B: Example of MSI-H where all markers are instable. C: Example of MSI-H where three of four markers are instable. The Journal of Molecular Diagnostics 2008 10, 154-159DOI: (10.2353/jmoldx.2008.070087) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Examples of allelic shift patterns in dinucleotide markers D2S1223 (A) and D17S250 (B) in an MSI-H sample (A), and an MSI-L (B) sample. N, normal tissue, T, tumor tissue. The Journal of Molecular Diagnostics 2008 10, 154-159DOI: (10.2353/jmoldx.2008.070087) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions