Frequent monitoring of Epstein-Barr virus DNA load in unfractionated whole blood is essential for early detection of posttransplant lymphoproliferative.

Slides:



Advertisements
Similar presentations
A lower risk of recurrent venous thrombosis in women compared with men is explained by sex-specific risk factors at time of first venous thrombosis in.
Advertisements

The Tale of two Herpes Viruses: CMV and EBV Sharon F. Chen, M.D., M.S. Hayley Gans, M.D. Pediatric Infectious Diseases Pediatric Infectious Diseases Program.
Characterization and treatment of chronic active Epstein- Barr virus disease: a 28-year experience in the United States by Jeffrey I. Cohen, Elaine S.
From: Treatment of Refractory Whipple Disease with Interferon-γ
Specificity of EAS FISH
Laboratory Assays for Epstein-Barr Virus-Related Disease
Circulating Epstein–Barr virus (EBV) in HIV-infected patients and its relation with primary brain lymphoma  María Dolores Fellner, Karina Durand, Rita.
Cell type specific infection of Epstein–Barr virus (EBV) in EBV-associated hemophagocytic lymphohistiocytosis and chronic active EBV infection  Yoshihito.
Severe human herpesvirus-8 primary infection in a renal transplant patient successfully treated with anti-CD20 monoclonal antibody by Olivier Thaunat,
Evaluation of the Epstein-Barr Virus R-Gene Quantification Kit in Whole Blood with Different Extraction Methods and PCR Platforms  Samira Fafi-Kremer,
Epstein-Barr Virus Strains With Latent Membrane Protein-1 Deletions: Prevalence in the Italian Population and High Association With Human Immunodeficiency.
Molecular Diagnosis of Epstein-Barr Virus-Related Diseases
by Yoko Otake, Sridharan Soundararajan, Tapas K. Sengupta, Ebenezer A
Dasatinib induces complete remission in a patient with primary cerebral involvement of B-cell chronic lymphocytic leukemia failing chemotherapy by Georg.
Anti-CD20 monoclonal antibody treatment in 6 patients with therapy-refractory chronic graft-versus-host disease by Marijke R. Canninga-van Dijk, Hanneke.
Reduced tumor load in peripheral blood after treatment with G-CSF and chemotherapy in children with tumors of the Ewing sarcoma family but not neuroblastoma.
Further Analysis of Interleukin-2 Receptor Subunit Expression on the Different Human Peripheral Blood Mononuclear Cell Subsets by Denis David, Lynda Bani,
Lytic Replication of Epstein-Barr Virus in the Peripheral Blood: Analysis of Viral Gene Expression in B Lymphocytes During Infectious Mononucleosis and.
Rearranged Ig Heavy Chain DNA Is Detectable in Cell-Free Blood Samples of Patients With B-Cell Neoplasia by N. Frickhofen, E. Müller, M. Sandherr, T. Binder,
by Andrew D. Redd, Ava Avalos, and Max Essex
by Thomas Rasmussen, Linda Jensen, Lone Honoré, and Hans Erik Johnsen
Maintenance of HCV-specific T-cell responses in antibody-deficient patients a decade after early therapy by Nasser Semmo, Michaela Lucas, George Krashias,
by Romina Gamberale, Jorge R. Geffner, Julieta Sanjurjo, Paula X
by Dennis Adeegbe, Robert B. Levy, and Thomas R. Malek
Serum Levels of Substance P Are Elevated in Patients With Sickle Cell Disease and Increase Further During Vaso-Occlusive Crisis by Lisa A. Michaels, Kwaku.
Long-term expansion of effector/memory Vδ2− γδ T cells is a specific blood signature of CMV infection by Vincent Pitard, David Roumanes, Xavier Lafarge,
Simultaneous Ex Vivo Expansion of Cytomegalovirus and Epstein-Barr Virus–Specific Cytotoxic T Lymphocytes Using B-Lymphoblastoid Cell Lines Expressing.
Characterization and treatment of chronic active Epstein-Barr virus disease: a 28-year experience in the United States by Jeffrey I. Cohen, Elaine S. Jaffe,
E. Bronska, J. Kalmusova, O. Dzupova, V. Maresova, P. Kriz, J. Benes 
Rapid Simultaneous Screening of Factor V Leiden and G20210A Prothrombin Variant by Multiplex Polymerase Chain Reaction on Whole Blood by Encarnación Gómez,
Early Cytomegalovirus Reactivation Leaves a Specific and Dynamic Imprint on the Reconstituting T Cell Compartment Long-Term after Hematopoietic Stem Cell.
Accurate quantification of minimal residual disease at day 15, by real-time quantitative polymerase chain reaction identifies also patients with B-precursor.
Figure 3 Immunohistochemical analyses of positive and negative Epstein-Barr virus (EBV) control tissues using immunostaining Immunohistochemical analyses.
Epstein–Barr virus (EBV) DNA in whole blood as a superior prognostic and monitoring factor than EBV-encoded small RNA in situ hybridization in diffuse.
Age-related Epstein-Barr virus (EBV)–associated B-cell lymphoproliferative disorders: comparison with EBV-positive classic Hodgkin lymphoma in elderly.
Epstein-Barr virus (EBV) reactivation is a frequent event after allogeneic stem cell transplantation (SCT) and quantitatively predicts EBV-lymphoproliferative.
Longitudinal dynamics of antigen-specific CD8+cytotoxic T lymphocytes following primary Epstein-Barr virus infection by Mandvi Bharadwaj, Scott R. Burrows,
Expansion of CD7low and CD7negative CD8 T-cell effector subsets in HIV-1 infection: correlation with antigenic load and reversion by antiretroviral treatment.
Human NK cells can control CMV infection in the absence of T cells
by Ineke J. M. ten Berge, and René A. W. van Lier
Treatment-related risk factors of inhibitor development in previously untreated patients with hemophilia A: the CANAL cohort study by Samantha C. Gouw,
Analysis of the Human Ferrochelatase Promoter in Transgenic Mice
Alicia K. Morgans, MD, Ran Reshef, MD, Donald E. Tsai, MD, PhD 
Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses by Cagan Gurer, Till.
Laboratory Assays for Epstein-Barr Virus-Related Disease
LEF-1 is a prosurvival factor in chronic lymphocytic leukemia and is expressed in the preleukemic state of monoclonal B-cell lymphocytosis by Albert Gutierrez,
Innovative Two-Step Negative Selection of Granulocyte Colony-Stimulating Factor–Mobilized Circulating Progenitor Cells: Adequacy for Autologous and Allogeneic.
Yang et al. SALL4 is a key regulator of survival and apoptosis in human leukemic cells. Blood. 2008;112(3):805–813. Blood Volume 114(14): October.
Successful treatment of posttransplantation lymphoproliferative disorder (PTLD) following renal allografting is associated with sustained CD8+ T-cell restoration.
Tumor Necrosis Factor Receptor-Associated Factor 1 Is Overexpressed in Reed-Sternberg Cells of Hodgkin’s Disease and Epstein-Barr Virus-Transformed Lymphoid.
Cytokine-Release Syndrome in Patients With B-Cell Chronic Lymphocytic Leukemia and High Lymphocyte Counts After Treatment With an Anti-CD20 Monoclonal.
Molecular and diagnostic clinical virology in real time
Application of the ELISPOT assay to the characterization of CD8+ responses to Epstein-Barr virus antigens by Jie Yang, Victor M. Lemas, Ian W. Flinn, Chris.
Molecular and diagnostic clinical virology in real time
Volume 20, Issue 5, Pages (May 2012)
Fatal Early-Onset Epstein-Barr Virus–Associated Posttransplantation Lymphoproliferative Disease after Successful Adult Dual-Unit Umbilical Cord Blood.
Molecular Therapy - Methods & Clinical Development
Volume 23, Issue 1, Pages (January 2015)
PPARδ Is a Type 1 IFN Target Gene and Inhibits Apoptosis in T Cells
Clonotypic heterogeneity in cutaneous T-cell lymphoma (mycosis fungoides) revealed by comprehensive whole-exome sequencing by Aishwarya Iyer, Dylan Hennessey,
The distribution of lamin C and emerin in lymphoblastoid cell lines.
Figure 1 Peripheral blood lymphocyte counts during dose titrationB-lymphocyte (CD19+; A) and total lymphocyte (CD45+; B) counts (cells/µL) in peripheral.
Improved Detection of the KIT D816V Mutation in Patients with Systemic Mastocytosis Using a Quantitative and Highly Sensitive Real-Time qPCR Assay  Thomas.
Reverse transcriptase polymerase chain reaction (RT-PCR) analysis of EBNA1 promoter usage in Epstein-Barr virus (EBV) associated disorders. Reverse transcriptase.
Rapid immune reconstitution of SCID-X1 canines after G-CSF/AMD3100 mobilization and in vivo gene therapy by Olivier Humbert, Frieda Chan, Yogendra S. Rajawat,
The Dispersal of Mucosal Memory B Cells
by Honglin Chen, Paul Smith, Richard F. Ambinder, and S. Diane Hayward
IFN-γ-producing T-cells in SLE patients and healthy controls upon EBV antigen stimulation. IFN-γ-producing T-cells in SLE patients and healthy controls.
Activated T-cells in SLE patients and healthy controls upon EBV antigen stimulation. Activated T-cells in SLE patients and healthy controls upon EBV antigen.
CD47 functions as a molecular switch for erythrocyte phagocytosis
Presentation transcript:

Frequent monitoring of Epstein-Barr virus DNA load in unfractionated whole blood is essential for early detection of posttransplant lymphoproliferative disease in high-risk patients by Servi J. C. Stevens, Erik A. M. Verschuuren, Inge Pronk, Wim van der Bij, Martin C. Harmsen, T. Hauw The, Chris J. L. M. Meijer, Adriaan J. C. van den Brule, and Jaap M. Middeldorp Blood Volume 97(5):1165-1171 March 1, 2001 ©2001 by American Society of Hematology

Experimental approach for EBV DNA load determination in whole blood and serum.Fresh whole blood or serum was lysed in guanidine isothiocyanate (GuSCN) buffer, and DNA was isolated by silica-based extraction. Experimental approach for EBV DNA load determination in whole blood and serum.Fresh whole blood or serum was lysed in guanidine isothiocyanate (GuSCN) buffer, and DNA was isolated by silica-based extraction. EBV status was assessed by qualitative EBNA-1 PCR, and EBV DNA in positive samples was quantified by quantitative competitive PCR. DNA quality was checked with β-globin PCR. Servi J. C. Stevens et al. Blood 2001;97:1165-1171 ©2001 by American Society of Hematology

EBV DNA load dynamics in unfractionated whole blood of PTLD patient nos. 1 and 2.(A) Patient no. 1 developed 3 episodes of PTLD after primary EBV infection, all preceded by increased EBV DNA loads. EBV DNA load dynamics in unfractionated whole blood of PTLD patient nos. 1 and 2.(A) Patient no. 1 developed 3 episodes of PTLD after primary EBV infection, all preceded by increased EBV DNA loads. (B) Patient no. 2 was converted from cyclosporine A to FK506 as therapy for chronic rejection, which was followed by dramatically increasing EBV DNA loads that fluctuated strongly. EBV+ plasmacytoma in the mouth was diagnosed approximately 300 days later. Black boxes indicate acyclovir treatment and white boxes indicate gancyclovir treatment. Servi J. C. Stevens et al. Blood 2001;97:1165-1171 ©2001 by American Society of Hematology

EBER-RISH and CD20 staining of PBMNCs obtained from PTLD patient no EBER-RISH and CD20 staining of PBMNCs obtained from PTLD patient no. 1 and a healthy EBV+ donor.(A) EBER-RISH shows numerous EBV+ B cells (indicated by arrows) in the peripheral blood of PTLD patient no. 1 at the period of the third PTLD diagnosis. EBER-RISH and CD20 staining of PBMNCs obtained from PTLD patient no. 1 and a healthy EBV+ donor.(A) EBER-RISH shows numerous EBV+ B cells (indicated by arrows) in the peripheral blood of PTLD patient no. 1 at the period of the third PTLD diagnosis. Insert shows closeup of EBER and CD20+ cell. (B) In the healthy donor, there was no EBER signal detected, thereby confirming the low EBV loads in peripheral blood of healthy individuals. Servi J. C. Stevens et al. Blood 2001;97:1165-1171 ©2001 by American Society of Hematology