Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos Lodovico Parmegiani, B.Sc., Antonio.

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Presentation transcript:

Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos Lodovico Parmegiani, B.Sc., Antonio Accorsi, B.Sc., Graciela Estela Cognigni, M.D., Silvia Bernardi, B.Sc., Enzo Troilo, B.Sc., Marco Filicori, M.D. Fertility and Sterility Volume 94, Issue 4, Pages 1525-1528 (September 2010) DOI: 10.1016/j.fertnstert.2009.05.089 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Measurement of LN2 contamination. (A) Total bacteria on tryptone soy agar, 24 hours. (B) Pseudomonas aeruginosa on cetrimide agar, 24 hours. (C) Escherichia coli on McConkey agar, 24 hours. (D) Aspergillus niger on Sabouraud dextrose agar, 120 hours. In each photograph, Petri dishes 1 and 2 show the growth of the microorganisms after contamination of each dewar with ATCC pure strains. Petri dish 3 shows the growth in the watery detritus after LN2 evaporation. Petri dish 4 shows the growth in the watery detritus after UV irradiation and LN2 evaporation. Fertility and Sterility 2010 94, 1525-1528DOI: (10.1016/j.fertnstert.2009.05.089) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions