Genome Architecture from a Different Angle Elizabeth H. Finn, Tom Misteli  Developmental Cell  Volume 41, Issue 1, Pages 3-4 (April 2017) DOI: 10.1016/j.devcel.2017.03.017 Copyright © 2017 Terms and Conditions

Figure 1 Schematics of Methodology of Genome Architecture Mapping and C-methods In genome architecture mapping: physically proximal regions are selected by taking a thin section of the nuclei; individual cells are selected via microdissection; whole genomes of each nuclear section are sequenced; and interaction frequency is approximated by the number of times two loci were found in the same slice. In C-methods: physically proximal regions are selected by crosslinking, digest, and subsequent ligation; the ligation junctions are then sequenced; and interaction frequency is approximated by the number of times a given ligation junction is observed. While individual cell-level data can be generated after FACS sorting, this is not routinely done in standard C-methods. Developmental Cell 2017 41, 3-4DOI: (10.1016/j.devcel.2017.03.017) Copyright © 2017 Terms and Conditions