Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol.

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Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol Biochem 2016;38:2015-2029 - DOI:10.1159/000445561 Fig. 1. Effect of HBO pretreatment on myocardial infarct size. Images represent the infarction area and risk area of myocardial tissue in the sham, IR, DIL, HBO and combination therapy (HBO + diltiazem) groups, respectively. Infarction area is pale [present as (WI/WLV) %], risk area is red [present as (WR/WLV) %] and the normal tissue is blue. The results are presented as the mean ± SD (n = 5). ∆P < 0.05 compared with sham group, * P < 0.05 compared with the IR group, #P < 0.05 compared with the DIL group. In the sham group, no infarction was observed in the myocardium. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol Biochem 2016;38:2015-2029 - DOI:10.1159/000445561 Fig. 2. Effect of HBO pretreatment on serum lipid peroxidation in MIRI rats. The results are presented as the mean ± SD (n = 10). ∆P < 0.05 compared with sham group, * P < 0.05 compared with the IR group, #P < 0.05 compared with the DIL group. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol Biochem 2016;38:2015-2029 - DOI:10.1159/000445561 Fig. 3. Effect of HBO pretreatment on myocardial enzyme activities in MIRI rats. The results are presented as the mean ± SD (n = 10). ∆P < 0.05 compared with sham group, * P < 0.05 compared with the IR group, #P < 0.05 compared with the DIL group. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol Biochem 2016;38:2015-2029 - DOI:10.1159/000445561 Fig. 4. Effect of HBO pretreatment on serum NO and eNOS levels in MIRI rats. The results are presented as the mean ± SD (n = 10). ∆P < 0.05 compared with sham group, * P < 0.05 compared with the IR group, #P < 0.05 compared with the DIL group. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol Biochem 2016;38:2015-2029 - DOI:10.1159/000445561 Fig. 5. Effect of HBO pretreatment on myocardial tissues Na+-K+-ATPase and Ca2+-Mg2+-ATPase levels in MIRI rats. The results are presented as the mean ± SD (n = 5). ∆P < 0.05 compared with sham group, * P < 0.05 compared with the IR group, #P < 0.05 compared with the DIL group. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol Biochem 2016;38:2015-2029 - DOI:10.1159/000445561 Fig. 6. Histologic photographs (400 ×) and pathological grade of rats with myocardial ischemia-reperfusion injury. Grade 0: cardiomyocytes are normal, no abnormal changes including myocardial cell edema (or vacuoles). Grade I: myocardial swelling, limited myocardial cell necrosis with scattered distribution. Grade II: myofiber disruptions, focal necrosis. Grade III: myocardial structural disorder, large scope of myocardial necrosis with edema and infiltration of inflammatory cells. * P < 0.05, significantly different from the IR group. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol Biochem 2016;38:2015-2029 - DOI:10.1159/000445561 Fig. 7. Immunohistochemical results of cardiac muscle under a light microscope in rats (400 ×). Left: Representative photographs displaying the number of positive cells (Bax, and Bcl-2). Right: Calculation of positive neurons (Bax, and Bcl-2; cells/mm2). Each column represents the mean ± SD (n = 8). Statistical analyses were performed using Wilcoxon's test. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol Biochem 2016;38:2015-2029 - DOI:10.1159/000445561 Fig. 8. Effect of HBO pretreatment on apoptosis-related protein and gene expression in MIRI rats. The results are presented as the mean ± SD (n = 5). ∆P < 0.05 compared with sham group, * P < 0.05 compared with the IR group, #P < 0.05 compared with the DIL group. © 2016 The Author(s) Published by S. Karger AG, Basel - CC BY-NC-ND 4.0