A bovine protocol for training professionals in preimplantation genetic diagnosis using polymerase chain reaction Carlos Gilberto Almodin, M.D., Ph.D.,

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Presentation transcript:

A bovine protocol for training professionals in preimplantation genetic diagnosis using polymerase chain reaction Carlos Gilberto Almodin, M.D., Ph.D., Antonio Fernandes Moron, M.D., Ph.D., Luiz Kulay, M.D., Ph.D., Vânia Cibele Minguetti-Câmara, M.Sc., Andréa Cristina Moraes, Ph.D., Maria Regina Torloni, M.D., M.Sc. Fertility and Sterility Volume 84, Issue 4, Pages 895-899 (October 2005) DOI: 10.1016/j.fertnstert.2005.02.051 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 PCR product after Pst I enzyme digestion. The results confirm that the amplified fragments originate from bovine X chromosomes. Slot 1: embryo 1 (female); slot 2: biopsy 1A (female); slot 3: embryo 2 (no amplification); slot 4: biopsy 2A (no amplification); slot 5: control without DNA; slot 6: embryo 3 (female); slot 7: biopsy 3A (female); slot 8: CF (control female, fragment of 344 bp); slot 9: CM (control male, one fragment of 344 bp and another of 103 bp); slot 10: no (control without DNA). Almodin. PGD training with bovine embryo. Fertil Steril 2005. Fertility and Sterility 2005 84, 895-899DOI: (10.1016/j.fertnstert.2005.02.051) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Nested PCR for sex determination in embryos/biopsies. Digested with Pst I. Slot 1: marker; slot 2:control male; slot 3: control female; slot 4: embryo 12 (male); slot 5: biopsy 12A (no amplification); slot 6: embryo 13 (female); slot 7: biopsy 13A (female); slot 8: embryo 14 (female); slot 9: biopsy 14A (female); slot 10: embryo 15 (female); slot 11: biopsy 15A (female); slot 12: embryo 16 (female); slot 13: biopsy 16A (female); slot 14: embryo 17 (male); slot 15: biopsy 17A (male). Almodin. PGD training with bovine embryo. Fertil Steril 2005. Fertility and Sterility 2005 84, 895-899DOI: (10.1016/j.fertnstert.2005.02.051) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions