Quantitative Chemical Analysis Daniel C. Harris Quantitative Chemical Analysis Seventh Edition Chapter 23 Chromatography Copyright © 2007 by W. H. Freeman and Company

Example: V1 = 100 mL, K = 3 (1) Extract with 500 mL And 5 times with 500 mL? (2) Extract 5 times with 100 mL

How to cleverly do multiple extractions: Move one phase past The other

Separation factor: γ = tr2/tr1

So you can measure the outcome in volume or in time

The ideal Gaussian peak

resolving two Gaussian peaks

Can you improve resolution by just using a longer column (to spread out the peaks further)? Not necessarily!

Therefore resolution improves with square root of the column length

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