HSP65-PRA identification of non-tuberculosis mycobacteria from 4892 samples suspicious for mycobacterial infections M. Saifi, E. Jabbarzadeh, A.R. Bahrmand,

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HSP65-PRA identification of non-tuberculosis mycobacteria from 4892 samples suspicious for mycobacterial infections M. Saifi, E. Jabbarzadeh, A.R. Bahrmand, A. Karimi, S. Pourazar, A. Fateh, M. Masoumi, E. Vahidi Clinical Microbiology and Infection Volume 19, Issue 8, Pages 723-728 (August 2013) DOI: 10.1111/j.1469-0691.2012.04005.x Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Dendrogram generated by the Gelcompar II software (Applied Maths) showing the relationship of mycobacterial isolates. The analysis of the bands generated was performed using the Dice coefficient and unweighted pair group method with arithmetic averages (UPGMA). TB, TB complex; A, atypical mycobacteria; Sp, sputum; S.F, synovial fluid; BAL, bronchio alveolar lavage; U, urine; N, negative; P, positive; R, resistant; S, sensitive; ETH, ethionamid; K, kanamycin; EMB, ethambutol; Rif, rifampin; St, streptomycin; INH, isoniazide. *HIV positive. Clinical Microbiology and Infection 2013 19, 723-728DOI: (10.1111/j.1469-0691.2012.04005.x) Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Lane 1: 439 bp PCR product of hsp65 gene. Lanes 2, 3, 5 and 6: RFLP patterns of TB complex strains with BstEII. Lanes 4,7 and 8: RFLP patterns of NTM strain with BstEII. Lane 9: molecular weight marker. Clinical Microbiology and Infection 2013 19, 723-728DOI: (10.1111/j.1469-0691.2012.04005.x) Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Lanes 1 and 2: RFLP patterns of M. tuberculosis strains with HaeIII. Lane 3: RFLP patterns of M. bovis strain. Lane 4: RFLP patterns of NTM strain. Lane 5: molecular weight marker. Clinical Microbiology and Infection 2013 19, 723-728DOI: (10.1111/j.1469-0691.2012.04005.x) Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions