Volume 62, Issue 1, Pages 237-244 (July 2002) Kidney Injury Molecule-1 (KIM-1): A novel biomarker for human renal proximal tubule injury  Won K. Han, Veronique Bailly, Rekha Abichandani, Ravi Thadhani, Joseph V. Bonventre  Kidney International  Volume 62, Issue 1, Pages 237-244 (July 2002) DOI: 10.1046/j.1523-1755.2002.00433.x Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 1 KIM-1 expression in a normal human kidney (A) and one with acute tubular necrosis; (B) human kidney paraffin sections are stained for KIM-1 using the AKG7 anti-human KIM-1 monoclonal antibody, and an ABC peroxidase detection system with DAB substrate (Vector). Normal kidney tissue was taken from a patient with renal cell carcinoma. Kidney International 2002 62, 237-244DOI: (10.1046/j.1523-1755.2002.00433.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 2 Western blot analysis of urine collected from patients with different forms of renal failure. Each lane represents protein immunoextracted from urines of patients without renal disease, with ischemic ATN, other forms of ARF (prerenal azotemia, contrast nephropathy, post-renal), or chronic diseases (systemic lupus erythematosus nephropathy, SLE; diabetic nephropathy, DM). After immunoextraction and PAGE, Western blot analysis was carried out with the AKG7 anti-KIM-1 antibody. Kidney International 2002 62, 237-244DOI: (10.1046/j.1523-1755.2002.00433.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 3 Comparison of urinary KIM-1 concentration in various forms of renal diseases. Scatterogram of absolute KIM-1 concentration is shown to discriminate the value of KIM-1 among the different groups of patients. Number of patients in each group is indicated above the scatterogram. Values shown are means ±(SE). Abbreviations are: ATN, acute tubular necrosis; Other ARF, acute renal failure with causes other than ischemia and contrast nephropathy. Kidney International 2002 62, 237-244DOI: (10.1046/j.1523-1755.2002.00433.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 4 KIM-1 expression in urine of patient after abdominal aortic aneurysm repair. Time course of KIM-1 expression in urine of a patient who underwent an elective repair of an abdominal aortic aneurysm. Sequential urine samples were collected every 6 hours from the onset of operation. Urine sediment was checked at every time point KIM-1 was measured. Kidney International 2002 62, 237-244DOI: (10.1046/j.1523-1755.2002.00433.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 5 Comparison of KIM-1 and other urinary proteins as biomarkers for tubule injury in various renal diseases. Urinary alkaline phosphatase, γ-glutamyltransferase, (γGT) and total protein were measured for comparison with urine KIM-1 as markers for ATN. Number of patients in each group is indicated above the top bars. Urinary biomarker concentrations are normalized to urine creatinine concentration. Kidney International 2002 62, 237-244DOI: (10.1046/j.1523-1755.2002.00433.x) Copyright © 2002 International Society of Nephrology Terms and Conditions